Structure of PDB 4h82 Chain D

Receptor sequence

>4h82D (length=160) Species: 9606 (Homo sapiens)

FEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRV
YSRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDEL
WSLGKGQGYSLFLVAAHQFGHALGLDHSSVPEALMYPMYRFTEGPPLHKD
DVNGIRHLYG

3D structure

• PDB: 4h82 Crystallization of bi-functional ligand protein complexes.
• Chain: D
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H226 Q227 H230 H236
• Catalytic site (residue number reindexed from 1): H117 Q118 H121 H127
• Enzyme Commision number: 3.4.24.35: gelatinase B.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	D	H175 D177 H190 H203	H66 D68 H81 H94
BS02	L29	D	L187 L188 A189 L222 V223 H226 Q227 H230 H236 Y245 P246 Y248	L78 L79 A80 L113 V114 H117 Q118 H121 H127 Y136 P137 Y139	BindingDB: IC50=5.5nM
BS03	ZN	D	H226 H230 H236	H117 H121 H127
BS04	CA	D	D182 G183 D185 G186 L187 D205 E208	D73 G74 D76 G77 L78 D96 E99
BS05	CA	D	D131 D206 E208	D22 D97 E99
BS06	CA	D	D165 G197 Q199 D201	D56 G88 Q90 D92

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0031012 extracellular matrix

External links

• PDB: RCSB:4h82, PDBe:4h82, PDBj:4h82
• PDBsum: 4h82
• PubMed: 23567804
• UniProt: P14780|MMP9_HUMAN Matrix metalloproteinase-9 (Gene Name=MMP9)