Structure of PDB 4guv Chain D

Receptor sequence

>4guvD (length=367) Species: 818 (Bacteroides thetaiotaomicron)

NLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYERDNDREARIFGGTLD
LHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVKPENRFD
NPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETA
DLVILANGGMSKVRKFVTDTEVEETGTFNIQADIHQPEINCPGFFQLCNG
NRLMASHQGNLLFANPNNNGALHFGISFKTPDEWQVDFQNRNSVVDFLLK
EFSDWDERYKELIHTTLSFVGLATRIFPLEKPWKSKRPLPITMIGDAAHL
MPPFAGQGVNSGLVDALILSDNLADGKFNSIEEAVKNYEQQMFIYGKEAQ
EESTQNEIEMFKPDFTF

3D structure

• PDB: 4guv Putative dioxygen-binding sites and recognition of tigecycline and minocycline in the tetracycline-degrading monooxygenase TetX.
• Chain: D
• Resolution: 2.73 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.231: tetracycline 11a-monooxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	D	G23 G25 P26 V27 E46 R47 L60 R117 R137 K138 L139 N168 G169 Q192 G310 D311 P318 G323 V324	G12 G14 P15 V16 E35 R36 L49 R106 R126 K127 L128 N157 G158 Q181 G295 D296 P303 G308 V309
BS02	XE	D	I237 F267 Y274 I278	I226 F252 Y259 I263
BS03	XE	D	N221 F239 E266 F267	N210 F228 E251 F252

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004497 monooxygenase activity
• GO:0071949 FAD binding

Biological Process

• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4guv, PDBe:4guv, PDBj:4guv
• PDBsum: 4guv
• PubMed: 23999299
• UniProt: Q93L51|TETX_BACT4 Flavin-dependent monooxygenase (Gene Name=tetX2)