Structure of PDB 4bed Chain D

Receptor sequence

>4bedD (length=1734) Species: 55429 (Megathura crenulata)

VMIRKDITQLDKRQQLSLVKALESMKADHSSDGFQAIASFHALPPLCPSP
AASKRFACCVHGMATFPQWHRLYTVQFQDSLRKHGAVVGLPYWDWTLPRS
ELPELLTVSTIHDPETGRDIPNPFIGSKIEFEGENVHTKRDINRDRLFQG
STKTHHNWFIEQALLALEQTNYCDFEVQFEIMHNGVHTWVGGKEPYGIGH
LHYASYDPLFYIHHSQTDRIWAIWQSLQRFRGLSGSEANCAVNLMKTPLK
PFSFGAPYNLNDHTHDFSKPEDTFDYQKFGYIYDTLEFAGWSIRGIDHIV
RNRQEHSRVFAGFLLEGFGTSATVDFQVCRTAGDCEDAGYFTVLGGEKEM
PWAFDRLYKYDITETLDKMNLRHDEIFQIEVTITSYDGTVLDSGLIPTPS
IIYDPAHHDISSHHLSLNKVRHDLSTLSERDIGSLKYALSSLQADTSADG
FAAIASFHGLPAKCNDSHNNEVACCIHGMPTFPHWHRLYTLQFEQALRRH
GSSVAVPYWDWTKPIHNIPHLFTDKEYYDVWRNKVMPNPFARGYVPSHDT
YTVRDVQEGLFHLTSTGEHSALLNQALLALEQHDYCDFAVQFEVMHNTIH
YLVGGPQVYSLSSLHYASYDPIFFIHHSFVDKVWAVWQALQEKRGLPSDR
ADCAVSLMTQNMRPFHYEINHNQFTKKHAVPNDVFKYELLGYRYDNLEIG
GMNLHEIEKEIKDKQHHVRVFAGFLLHGIRTSADVQFQICKTSEDCHHGG
QIFVLGGTKEMAWAYNRLFKYDITHALHDAHITPEDVFHPSEPFFIKVSV
TAVNGTVLPASILHAPTIIYEPGLDHHEDHHSSSMAGHGVRKEINTLTTA
EVDNLKDAMRAVMADHGPNGYQAIAAFHGNPPMCPMPDGKNYSCCTHGMA
TFPHWHRLYTKQMEDALTAHGARVGLPYWDGTTAFTALPTFVTDEEDNPF
HHGHIDYLGVDTTRSPRDKLFNDPERGSESFFYRQVLLALEQTDFCQFEV
QFEITHNAIHSWTGGLTPYGMSTLEYTTYDPLFWLHHANTDRIWAIWQAL
QEYRGLPYDHANCEIQAMKRPLRPFSDPINHNAFTHSNAKPTDVFEYSRF
NFQYDNLRFHGMTIKKLEHELEKQKEEDRTFAAFLLHGIKKSADVSFDVC
NHDGECHFAGTFAILGGEHEMPWSFDRLFRYDITQVLKQMHLEYDSDFTF
HMRIIDTSGKQLPSDLIKMPTVEHSPGGKHHEKHHEDHHEDILVRKNIHS
LSHHEAEELRDALYKLQNDESHGGYEHIAGFHGYPNLCPEKGDEKYPCCV
HGMSIFPHWHRLHTIQFERALKKHGSHLGIPYWDWTQTISSLPTFFADSG
NNNPFFKYHIRSINQDTVRDVNEAIFQQTKFGEFSSIFYLALQALEEDNY
CDFEVQYEILHNEVHALIGGAEKYSMSTLEYSAFDPYFMIHHASLDKIWI
IWQELQKRRVKPAHAGSCAGDIMHVPLHPFNYESVNNDDFTRENSLPNAV
VDSHRFNYKYDNLNLHGHNIEELEEVLRSLRLKSRVFAGFVLSGIRTTAV
VKVYIKSGTDSDDEYAGSFVILGGAKEMPWAYERLYRFDITETVHNLNLT
DDHVKFRFDLKKYDHTELDASVLPAPIIVRRPNNAVFDIIEIPIGKDVNL
PPKVVVKRGTKIMFMSVDEAVTTPMLNLGSYTAMFKCKVPPFSFHAFELG
KMYSVESGDYFMTASTTELCNDNNLRIHVHVDDE

3D structure

• PDB: 4bed Keyhole Limpet Hemocyanin: 9-A Cryoem Structure and Molecular Model of the Klh1 Didecamer Reveal the Interfaces and Intricate Topology of the 160 Functional Units.
• Chain: D
• Resolution: 9.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H2122 H2141 H2150 H2670 H2674 L2688 H2701
• Catalytic site (residue number reindexed from 1): H458 H477 H486 H1006 H1010 L1024 H1037

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CUO	D	H1705 H1725 F1730 H1734 H1847 H1851 H1878	H41 H61 F66 H70 H183 H187 H214
BS02	CUO	D	H2122 H2141 H2150 H2260 H2264 H2291	H458 H477 H486 H596 H600 H627
BS03	CUO	D	H2542 H2561 H2570 H2670 H2674 H2701	H878 H897 H906 H1006 H1010 H1037
BS04	CUO	D	H2946 H2965 H2974 H3075 H3079 H3106	H1282 H1301 H1310 H1411 H1415 H1442

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:4bed, PDBe:4bed, PDBj:4bed
• PDBsum: 4bed
• PubMed: 19013468
• UniProt: Q10583|HCY1_MEGCR Hemocyanin 1 (Gene Name=KLH1)