Structure of PDB 3sbr Chain D

Receptor sequence

>3sbrD (length=588) Species: 316 (Stutzerimonas stutzeri)

QAVKESKQKIHVGPGELDDYYGFWSGGHQGEVRVLGVPSMRELMRIPVFN
VDSATGWGLTNESRHIMGDSAKFLNGDCHHPHISMTDGKYDGKYLFINDK
ANSRVARIRLDIMKCDKMITVPNVQAIHGLRLQKVPHTKYVFANAEFIIP
HPNDGKVFDLQDENSYTMYNAIDAETMEMAFQVIVDGNLDNTDADYTGRF
AAATCYNSEKAFDLGGMMRNERDWVVVFDIHAVEAAVKAGDFITLGDSKT
PVLDGRKKDGKDSKFTRYVPVPKNPHGCNTSSDGKYFIAAGKLSPTCSMI
AIDKLPDLFAGKLADPRDVIVGEPELGLGPLHTTFDGRGNAYTTLFIDSQ
VVKWNMEEAVRAYKGEKVNYIKQKLDVHYQPGHLHASLCETNEADGKWLV
ALSKFSKDRFLPVGPLHPENDQLIDISGDEMKLVHDGPTFAEPHDCIMAR
RDQIKTKKIWDRNDPFFAPTVEMAKKDGINLDTDNKVIRDGNKVRVYMTS
MAPAFGVQEFTVKQGDEVTVTITNIDQIEDVSHGFVVVNHGVSMEISPQQ
TSSITFVADKPGLHWYYCSWFCHALHMEMVGRMMVEPA

3D structure

• PDB: 3sbr N2O binding at a [4Cu:2S] copper-sulphur cluster in nitrous oxide reductase.
• Chain: D
• Resolution: 2.24 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M551 P553
• Catalytic site (residue number reindexed from 1): M501 P503
• Enzyme Commision number: 1.7.2.4: nitrous-oxide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CUA	D	C618 W620 C622 H626 M629	C568 W570 C572 H576 M579
BS02	CUK	D	H129 H130 H178 H326 H382 H433 K454 H494	H79 H80 H128 H276 H332 H383 K404 H444
BS03	CA	D	Y256 E259 M267 D273 N324	Y206 E209 M217 D223 N274
BS04	N2O	D	F621 M627	F571 M577

Gene Ontology

Molecular Function

• GO:0004129 cytochrome-c oxidase activity
• GO:0005507 copper ion binding
• GO:0005509 calcium ion binding
• GO:0050304 nitrous-oxide reductase activity

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3sbr, PDBe:3sbr, PDBj:3sbr
• PDBsum: 3sbr
• PubMed: 21841804
• UniProt: P19573|NOSZ_STUST Nitrous-oxide reductase (Gene Name=nosZ)