Structure of PDB 3mzs Chain D

Receptor sequence
>3mzsD (length=470) Species: 9913 (Bos taurus) [Search protein sequence]
TPRPYSEIPSPGDNGWLNLYHFWREKGSQRIHFRHIENFQKYGPIYREKL
GNLESVYIIHPEDVAHLFKFEGSYPERYDIPPWLAYHRYYQKPIGVLFKK
SGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVSLLHKRIKQQGSGK
FVGDIKEDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKMFHT
SVPLLNVPPELYRLFRTKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTE
FRNYPGILYCLLKSEKMLLEDVKANITEMLAGGVNTTSMTLQWHLYEMAR
SLNVQEMLREEVLNARRQAEGDISKMLQMVPLLKASIKETLRLHPISVTL
QRYPESDLVLQDYLIPAKTLVQVAIYAMGRDPAFFSSPDKFDPTRWLSKD
KDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEMQHIGDVD
TIFNLILTPDKPIFLVFRPF
3D structure
PDB3mzs Structural Basis for Three-step Sequential Catalysis by the Cholesterol Side Chain Cleavage Enzyme CYP11A1.
ChainD
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T291 F416 C423
Catalytic site (residue number reindexed from 1) T286 F411 C418
Enzyme Commision number 1.14.15.6: cholesterol monooxygenase (side-chain-cleaving).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM D R82 V101 R113 G287 T291 R357 G415 F416 G417 R421 C423 V424 G425 R77 V96 R108 G282 T286 R352 G410 F411 G412 R416 C418 V419 G420
BS02 HC9 D W88 S352 V353 Q356 W83 S347 V348 Q351
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008386 cholesterol monooxygenase (side-chain-cleaving) activity
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
Biological Process
GO:0006694 steroid biosynthetic process
GO:0006700 C21-steroid hormone biosynthetic process
GO:0006704 glucocorticoid biosynthetic process
GO:0008203 cholesterol metabolic process
GO:0008207 C21-steroid hormone metabolic process
GO:0034650 cortisol metabolic process
GO:0042359 vitamin D metabolic process
GO:0042446 hormone biosynthetic process
GO:0071375 cellular response to peptide hormone stimulus
Cellular Component
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3mzs, PDBe:3mzs, PDBj:3mzs
PDBsum3mzs
PubMed21159775
UniProtP00189|CP11A_BOVIN Cholesterol side-chain cleavage enzyme, mitochondrial (Gene Name=CYP11A1)

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