Structure of PDB 3htx Chain D

Receptor sequence

>3htxD (length=795) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]

HTPTPKAIIHQKFGAKASYTVEEVHDSSQSGCPGLAIPQKGPCLYRCHLQ
LPEFSVVSNVFKKKKDSEQSAAELALDKLGIRPQNDDLTVDEARDEIVGR
IKYIFSDEFLSAEHPLGAHLRAALRRDGERCGSVPVSVIATVDAKINSRC
KIINPSVESDPFLAISYVMKAAAKLADYIVASPHGLRRKNAYPSEIVEAL
ATHVSDSLHSREVAAVYIPCIDEEVVELDTLYISSNRHYLDSIAERLGLK
DGNQVMISRMFGKASCGSECRLYSEIPKKYLDNSSIVKSRNARASYICGQ
DIHGDAILASVGYRWKSDDLDYDDVTVNSFYRICCGMSPNGIYKISRQAV
IAAQLPFAFTTKSNWRGPLPREILGLFCHQHRLAEPILSSSRCEVKIFTK
SQDLVLECSPRKFYEKENDAIQNASLKALLWFSKFFADLSPNVFAAPPSS
ESKEKRVQSITNGSVVSICYSLSLAVDPEYESSVEPIESNEEIEFEVGTG
SMNPHIESEVTQMTVGEYASFKMTPPDAAEALILAVGSDTVRIRSLLSER
PCLNYNILLLGVKGPSEERMEAAFFKPPLSKQRVEYALKHIRESSASTLV
DFGCGSGSLLDSLLDYPTSLQTIIGVDISPKGLARAAKMLHVKLNKEACN
VKSATLYDGSILEFDSRLHDVDIGTCLEVIEHMEEDQACEFGEKVLSLFH
PKLLIVSTPNYEFNTILQRSTLPKFRNHDHKFEWTREQFNQWASKLGKRH
NYSVEFSGVGGSGEVEPGFASQIAIFRREESSMQPYKVIWEWKKE

3D structure

PDB

3htx Structural insights into mechanisms of the small RNA methyltransferase HEN1.

Chain

Resolution

3.1 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.386: small RNA 2'-O-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	D	P11 I14 Q17 Q45 G47 K71 Q75 W333 E390 E689 A691 F692 P696 L697 S698 K699 R701 E796 H800 L835 Q836 R856 H860	P5 I8 Q11 Q39 G41 K65 Q69 W315 E372 E571 A573 F574 P578 L579 S580 K581 R583 E678 H682 L717 Q718 R726 H730
BS02	rna	D	K18 Y51 K68 K69 K70 Y109 W333 H397 K477 E478 R687 E689 S747 H800 N857	K12 Y45 K62 K63 K64 Y103 W315 H379 K416 E417 R569 E571 S629 H682 N727
BS03	MG	D	E796 E799 H800 H860	E678 E681 H682 H730
BS04	SAH	D	G721 D745 I746 S778 I779 L795 E796	G603 D627 I628 S660 I661 L677 E678

Gene Ontology

	Molecular Function
GO:0003723	RNA binding
GO:0003755	peptidyl-prolyl cis-trans isomerase activity
GO:0008168	methyltransferase activity
GO:0008171	O-methyltransferase activity
GO:0008173	RNA methyltransferase activity
GO:0046872	metal ion binding
GO:0090486	small RNA 2'-O-methyltransferase activity

	Biological Process
GO:0001510	RNA methylation
GO:0009616	RNAi-mediated antiviral immune response
GO:0009909	regulation of flower development
GO:0010093	specification of floral organ identity
GO:0010267	ta-siRNA processing
GO:0010305	leaf vascular tissue pattern formation
GO:0010589	leaf proximal/distal pattern formation
GO:0016070	RNA metabolic process
GO:0031047	regulatory ncRNA-mediated gene silencing
GO:0032259	methylation
GO:0035196	miRNA processing
GO:0035279	miRNA-mediated gene silencing by mRNA destabilization

	Cellular Component
GO:0005634	nucleus
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:3htx, PDBe:3htx, PDBj:3htx
PDBsum	3htx
PubMed	19812675
UniProt	Q9C5Q8\|HEN1_ARATH Small RNA 2'-O-methyltransferase (Gene Name=HEN1)

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