Structure of PDB 3gv5 Chain D

Receptor sequence

>3gv5D (length=383) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCQDGRKPHTVRLIIRRYSSGRESRQCPIPSHVIQKLNYDVMTP
MVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKA

3D structure

• PDB: 3gv5 Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota
• Chain: D
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	L123 E127 K207 G241 I242 G243 K245 T246 E358 S359 R360 Q361	L98 E102 K182 G216 I217 G218 K220 T221 E329 S330 R331 Q332
BS02	dna	D	Q59 K60 Y61 L62 E97 L99 Y244 Q300 S301 S303 E305 S307 R347 R357	Q34 K35 Y36 L37 E72 L74 Y219 Q275 S276 S278 E280 S282 R322 R328
BS03	CA	D	D34 L35 D126	D9 L10 D101
BS04	CA	D	D34 E127	D9 E102
BS05	ADI	D	L35 D36 C37 F38 V64 T65 Y68 R71 K77	L10 D11 C12 F13 V39 T40 Y43 R46 K52

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3gv5, PDBe:3gv5, PDBj:3gv5
• PDBsum: 3gv5
• PubMed: 19440206
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)