Structure of PDB 3g1u Chain D

Receptor sequence

>3g1uD (length=418) Species: 5664 (Leishmania major)

DYKVKDISLAEWGRKAIELAENEMPGLMELRREYGPSQPLKGAKIAGCLH
MTVQTAVLIETLKALGAELRWSSCNIFSTQDNAAAAIAKTGVPVFAWKGE
TDEEYEWCIAQTVKGFSGDGLPNMILDDGGDLTNLVIDRYPELVPKIFGI
SEETTTGVKNLYKRLSKGNLPISAINVNDSSKFDNLYGCRESLVDGIKRA
TDVMIAGKTCCVCGYGDVGKGCAAALRAFGARVVVTEVDPINALQASMEG
YQVALVEDVMADAHIFVTTTGNDDIITSDHFPHMRDDAIVCNIGHFDTEI
QVGWLEANAKEHVEIKPQVDRYTMENGRHIILLAKGRLVNLGCASGHPSF
VMSNSFTNQVLAQIELWSNRDNGKYPRGDKAGVFFLPKALDEKVAALHLA
HVGAKLTKLTPKQAEYIN

3D structure

• PDB: 3g1u Crystal structure of Leishmania major S-adenosylhomocysteine hydrolase
• Chain: D
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H52 S75 S80 D130 E155 N180 D189 N190 C194 H300 H352 S360 Q364
• Catalytic site (residue number reindexed from 1): H50 S73 S78 D128 E153 N178 D184 N185 C189 H295 H347 S355 Q359
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADN	D	L51 H52 T54 Q56 D130 E155 T156 D189 H300 L346 H352 M357	L49 H50 T52 Q54 D128 E153 T154 D184 H295 L341 H347 M352
BS02	NAD	D	T156 T157 T158 N190 G221 V223 E242 V243 N247 T274 T275 N277 I280 I298 G299 N345 H352	T154 T155 T156 N185 G216 V218 E237 V238 N242 T269 T270 N272 I275 I293 G294 N340 H347

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3g1u, PDBe:3g1u, PDBj:3g1u
• PDBsum: 3g1u
• UniProt: Q4Q124