Structure of PDB 3flk Chain D

Receptor sequence
>3flkD (length=359) Species: 303 (Pseudomonas putida) [Search protein sequence]
SFRIAAIPGDGIGLEVLPEGIRVLEAAALKHGLALEFDTFEWASCDYYLQ
HGKMMPDDWAEQLKQYDAIYFGAVGWPDKVPDHISLWGSLLKFRREFDQY
VNIRPVRLFPGVPCALANRKVGDIDFVVVRENTEGEYSSLGGIMFENTEN
EIVIQESIFTRRGVDRILKYAFDLAEKRERKHVTSATKSNGMAISMPYWD
KRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVASNLFGDILSDL
GPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWS
GALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDMGGTLSTQQVGA
AISDTLARL
3D structure
PDB3flk Structural characterization of tartrate dehydrogenase: a versatile enzyme catalyzing multiple reactions.
ChainD
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y141 K192 D225 D250 D254
Catalytic site (residue number reindexed from 1) Y137 K188 D221 D245 D249
Enzyme Commision number 1.1.1.83: D-malate dehydrogenase (decarboxylating).
1.1.1.93: tartrate dehydrogenase.
4.1.1.73: tartrate decarboxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAI D N194 I226 R230 N190 I222 R226
BS02 NAI D I16 A77 V78 G79 D86 L94 I263 H285 G286 S287 A288 D290 I291 A297 N298 D343 I12 A73 V74 G75 D82 L90 I258 H280 G281 S282 A283 D285 I286 A292 N293 D338
BS03 OXL D R98 R108 R134 D250 R94 R104 R130 D245
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0009027 tartrate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016829 lyase activity
GO:0046553 D-malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0046872 metal ion binding
GO:0050319 tartrate decarboxylase activity
GO:0051287 NAD binding
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Cellular Component
External links
PDB RCSB:3flk, PDBe:3flk, PDBj:3flk
PDBsum3flk
PubMed20516620
UniProtQ51945|TTUC_PSEPU Tartrate dehydrogenase/decarboxylase

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