Structure of PDB 3erc Chain D

Receptor sequence

>3ercD (length=445) Species: 10254 (Vaccinia virus WR)

NITLKIIETYLGRVPSVNEYHMLKSQARNIQKITVFNKDIFVSLVKKNKK
RFFSDVNTSASEIKDRILSYFSKQTQTYNIGKLFTIIELQSVLVTTYTDI
LGVLTINVTSMEELARDMLNSMNVAVVSSLVKNVNKLMEEYLRRHNKSCI
CYGSYSLYLINPNIRYGDIDILQTNSRTFLIDLAFLIKFITGNNIILSKI
PYLRNYMVIKDENDNHIIDSFNIRQDTMNVVPKIFIDNIYIVDPTFQLLN
MIKMFSQIDRLEDLSKDPEKFNARMATMLEYVRYTHGIVFDGKRNNMPMK
CIIDENNRIVTVTTKDYFSFKKCLVYLDENVLSSDILDLNADTSCDFESV
TNSVYLIHDNIMYTYFSNTILLSDKGKVHEISARGLCAHILLYQMLTSGE
YKQCLSDLLNSMMNRDKIPIYSHTERDKKPGRHGFINIEKDIIVF

3D structure

• PDB: 3erc Polymerase Translocation with Respect to Single-Stranded Nucleic Acid: Looping or Wrapping of Primer around a Poly(A) Polymerase
• Chain: D
• Resolution: 3.21 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.19: polynucleotide adenylyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	Y236 L237 F255 H467	Y202 L203 F221 H433
BS02	dna	D	F47 N48 D50 I51 F52 S54 L55 K58 T109 G113 T116 I117	F36 N37 D39 I40 F41 S43 L44 K47 T98 G102 T105 I106
BS03	U	D	K93 I476	K82 I442
BS04	3AT	D	G187 S188 D202 N284 M288 K304 R308 N402	G153 S154 D168 N250 M254 K270 R274 N368
BS05	CA	D	D202 D204 D253	D168 D170 D219
BS06	CA	D	D202 D204	D168 D170

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:1990817 poly(A) RNA polymerase activity

Biological Process

• GO:0006397 mRNA processing

External links

• PDB: RCSB:3erc, PDBe:3erc, PDBj:3erc
• PDBsum: 3erc
• PubMed: 19446524
• UniProt: P23371|PAP1_VACCW Poly(A) polymerase catalytic subunit (Gene Name=OPG063)