Structure of PDB 3dla Chain D

Receptor sequence

>3dlaD (length=657) Species: 1773 (Mycobacterium tuberculosis)

SMNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAV
FPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRH
RHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIG
GADVAFGTDLLFAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLAN
LSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTM
IWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHREL
TESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVS
GLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFAL
PGFNNAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFEN
VQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNVNA
GVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEEAKVGPFALQDFSLF
QVLRYGFRPSKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQ
IFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPSDMSARIWLDQI
DREVPKG

3D structure

• PDB: 3dla Regulation of active site coupling in glutamine-dependent NAD(+) synthetase.
• Chain: D
• Resolution: 2.35 Å

Enzymatic activity

• Enzyme Commision number: 6.3.5.1: NAD(+) synthase (glutamine-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	DND	D	R354 L358 N471 G475 I476 H501	R355 L359 N465 G469 I470 H495
BS02	ONL	D	F130 C176 E177 R209	F131 C177 E178 R210
BS03	DND	D	E455 N456 W490 S491 T492 Y493 D497 F634 K635	E449 N450 W484 S485 T486 Y487 D491 F612 K613

Gene Ontology

Molecular Function

• GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
• GO:0004359 glutaminase activity
• GO:0005524 ATP binding
• GO:0008795 NAD+ synthase activity
• GO:0016874 ligase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009435 NAD biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:3dla, PDBe:3dla, PDBj:3dla
• PDBsum: 3dla
• PubMed: 19270703
• UniProt: P9WJJ3|NADE_MYCTU Glutamine-dependent NAD(+) synthetase (Gene Name=nadE)