Structure of PDB 2yoo Chain D

Receptor sequence
>2yooD (length=394) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence]
TRPDVDLVNGMFYADGGAREAYRWMRANEPVFRDRNGLAAATTYQAVLDA
ERNPELFSSTGGIRPDQPGMPYMIDMDDPQHLLRRKLVNAGFTRKRVMDK
VDSIGRLCDTLIDAVCERGECDFVRDIAAPLPMAVIGDMLGVLPTERDML
LKWSDDLVCGLSSHVDEAAIQKLMDTFAAYTEFTKDVITKRRAEPTDDLF
SVLVNSEVEGQRMSDDEIVFETLLILIGGDETTRHTLSGGTEQLLRHRDQ
WDALVADVDLLPGAIEEMLRWTSPVKNMCRTLTADTVFHGTELRAGEKIM
LMFESANFDESVFGDPDNFRIDRNPNSHVAFGFGTHFCLGNQLARLELRL
MTERVLRRLPDLRLADDAPVPLRPANFVSGPESMPVVFTPSAPV
3D structure
PDB2yoo A Highly Conserved Mycobacterial Cholesterol Catabolic Pathway.
ChainD
Resolution1.69 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C164 G233 E236 T237 T238 C343 L344 G345 E352 V383
Catalytic site (residue number reindexed from 1) C159 G228 E231 T232 T233 C338 L339 G340 E347 V378
Enzyme Commision number 1.14.15.28: cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM D E56 I79 H86 R90 F97 G233 G234 T237 T238 V280 M283 R285 A335 F336 G337 F338 H341 C343 L344 G345 E51 I74 H81 R85 F92 G228 G229 T232 T233 V275 M278 R280 A330 F331 G332 F333 H336 C338 L339 G340
BS02 K2B D M179 F182 L229 I232 F382 M174 F177 L224 I227 F377 MOAD: Kd=0.52uM
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008395 steroid hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0036199 cholest-4-en-3-one 26-monooxygenase activity
GO:0046872 metal ion binding
Biological Process
GO:0006707 cholesterol catabolic process
GO:0008203 cholesterol metabolic process
GO:0016042 lipid catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:2yoo, PDBe:2yoo, PDBj:2yoo
PDBsum2yoo
PubMed23489718
UniProtA0R4Q6|CP142_MYCS2 Steroid C26-monooxygenase (Gene Name=cyp142)

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