Structure of PDB 2vnu Chain D

Receptor sequence

>2vnuD (length=694) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

DFTFPEYYSTARVMGGLKNGVLYQGNIQISEYNFLEGSVSLPRFSKPVLI
VGQKNLNRAFNGDQVIVELLPQSEWKAPSSIVLDSEHFDIQPTAKVVYIQ
RRSWRQYVGQLAPSSVDPQSSSTQNVFVILMDKCLPKVRIRTRRAAELLD
KRIVISIDSWPTTHKYPLGHFVRDLGTIESAQAETEALLLEHDVEYRPFS
KKVLECLPAEGHDWKAPTKLDDPEAVSKDPLLTKRKDLRDKLICSIDPPG
CVDINDALHAKKLPNGNWEVGVHIADVTHFVKPGTALDAEGAARGTSVYL
VDKRIDMLPMLLGTDLCSLKPYVDRFAFSVIWELDDSANIVNVNFMKSVI
RSREAFSYEQAQLRIDDKTQNDELTMGMRALLKLSVKLKQKRLEAGALNL
ASPEVKVHMDSETSDPNEVEIKKLLATNSLVEEFMLLANISVARKIYDAF
PQTAMLRRHAAPPSTNFEILNEMLNTRKNMSISLESSKALADSLDRCVDP
EDPYFNTLVRIMSTRCMMAAQYFYSGAYSYPDFRHYGLAVDIYTHFTSPI
RRYCDVVAHRQLAGAIGYEPLSLTHRDKNKMDMICRNINRKHRNAQFAGR
ASIEYYVGQVMRNNESTETGYVIKVFNNGIVVLVPKFGVEGLIRLDNLTE
DPNSAAFDEVEYKLTFVPTNSDKPRDVYVFDKVEVQVRKRKAEL

3D structure

PDB

2vnu Structure of the Active Subunit of the Yeast Exosome Core, Rrp44: Diverse Modes of Substrate Recruitment in the Rnase II Nuclease Family

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D543 D549 N551 D552
Catalytic site (residue number reindexed from 1)	D247 D253 N255 D256
Enzyme Commision number	3.1.13.- 3.1.26.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	N277 Q279 S291 D543 P544 C547 D549 N551 D552 Y595 Y654 L696 A697 E700 E728 M731 R753 H755 M813 M814 A815 A816 H831 L834 Y839 H841 T843 R847 R896	N26 Q28 S40 D247 P248 C251 D253 N255 D256 Y299 Y358 L400 A401 E404 E432 M435 R457 H459 M517 M518 A519 A520 H535 L538 Y543 H545 T547 R551 R600
BS02	MG	D	D543 D552	D247 D256

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0004540	RNA nuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:2vnu, PDBe:2vnu, PDBj:2vnu
PDBsum	2vnu
PubMed	18374646
UniProt	Q08162\|RRP44_YEAST Exosome complex exonuclease DIS3 (Gene Name=DIS3)

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