Structure of PDB 2ldb Chain D

Receptor sequence

>2ldbD (length=301) Species: 1422 (Geobacillus stearothermophilus)

MKNNGGARVVVIGAGFVGASYVFALMNQGIADEIVLIDANESKAIGDAMD
FNHGKVFAPKPVDIWDYDDCRDADLVVICAGALDLVDKNIAIFRSIVESV
MASGFQGLFLVATNPVDILTYATWKFSGLPHERVIGSGTILDTARFRFLL
GEYFSVAPQNVHAYIIGEHGDTELPVWSQAYIGVMPIRKLVESKDLERIF
VNVRDAAYQIIEKKGATYYGIAMGLARVTRAILHNENAILTVSAYLDGLY
GERDVYIGVPAVINRNGIREVIEIELNDDEKNRFHHSAATLKSVLARAFT
R

3D structure

• PDB: 2ldb Structure determination and refinement of Bacillus stearothermophilus lactate dehydrogenase.
• Chain: D
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D166 R169 H193
• Catalytic site (residue number reindexed from 1): D142 R145 H169
• Enzyme Commision number: 1.1.1.27: L-lactate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	D	R171 Q183 H186	R147 Q159 H162
BS02	SO4	D	L165 R169 H193 T247	L141 R145 H169 T217
BS03	SO4	D	G43 A45 D46	G29 A31 D32
BS04	NAD	D	G29 F30 V31 D52 A53 A96 A136 T137 N138 H193 I251	G15 F16 V17 D38 A39 A80 A112 T113 N114 H169 I221

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0051287 NAD binding

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006096 glycolytic process
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2ldb, PDBe:2ldb, PDBj:2ldb
• PDBsum: 2ldb
• PubMed: 2330370
• UniProt: P00344|LDH_GEOSE L-lactate dehydrogenase (Gene Name=ldh)