Structure of PDB 2jif Chain D

Receptor sequence

>2jifD (length=381) Species: 9606 (Homo sapiens)

APLQTFTDEEMMIKSSVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQG
LMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIR
KHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVL
NGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPEN
KLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQM
LGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTY
NAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVE
KYFRDAKIGTIYEGASNIQLNTIAKHIDAEY

3D structure

• PDB: 2jif Crystal Structure of Human Short-Branched Chain Acyl-Coa Dehydrogenase
• Chain: D
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L176 S177 G293 E414 K426
• Catalytic site (residue number reindexed from 1): L125 S126 G242 E363 K375
• Enzyme Commision number: 1.3.8.5: short-chain 2-methylacyl-CoA dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	D	R319 F322 L326 F329 L332 E387 W388 G391	R268 F271 L275 F278 L281 E336 W337 G340
BS02	FAD	D	F174 L176 S177 G182 S183 W207 I208 S209 K252 N418 I419	F123 L125 S126 G131 S132 W156 I157 S158 K201 N367 I368
BS03	COS	D	S183 S185 F186 Y229 Y283 I287 L290 N291 R294 Y413 E414 G415 I419	S132 S134 F135 Y178 Y232 I236 L239 N240 R243 Y362 E363 G364 I368

Gene Ontology

Molecular Function

• GO:0003995 acyl-CoA dehydrogenase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0050660 flavin adenine dinucleotide binding

External links

• PDB: RCSB:2jif, PDBe:2jif, PDBj:2jif
• PDBsum: 2jif
• UniProt: P45954|ACDSB_HUMAN Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (Gene Name=ACADSB)