Structure of PDB 2isj Chain D

Receptor sequence
>2isjD (length=219) Species: 382 (Sinorhizobium meliloti) [Search protein sequence]
LTAAGAFSSDERAAVYRAIETRRDVRDEFLPEPLSEELIARLLGAAHQAP
SVGFMQPWNFVLVRQDETREKVWQAFQRANDEAAEMFSGERQAKYRSLKL
EGIRKAPLSICVTCDRTRGGAVVLGRTHNPQMDLYSTVCAVQNLWLAARA
EGVGVGWVSIFHESEIKAILGIPDHVEIVAWLCLGFVDRLYQEPELAAKG
WRQRLPLEDLVFEEGWGVR
3D structure
PDB2isj BluB cannibalizes flavin to form the lower ligand of vitamin B12.
ChainD
Resolution2.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.13.11.79: aerobic 5,6-dimethylbenzimidazole synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FMN D P58 S59 V60 M140 S144 P50 S51 V52 M132 S136 MOAD: Ki=231uM
BS02 FMN D R30 R31 D32 R34 L108 W165 V166 S167 P202 L204 R22 R23 D24 R26 L100 W157 V158 S159 P194 L196 MOAD: Ki=231uM
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0102919 5,6-dimethylbenzimidazole synthase activity
Biological Process
GO:0009236 cobalamin biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:2isj, PDBe:2isj, PDBj:2isj
PDBsum2isj
PubMed17377583
UniProtQ92PC8|BLUB_RHIME 5,6-dimethylbenzimidazole synthase (Gene Name=bluB)

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