Structure of PDB 2gie Chain D

Receptor sequence
>2gieD (length=256) Species: 727 (Haemophilus influenzae) [Search protein sequence]
SFIKPIYQDINSILIGQKVKRPKSGTLSGHAAGEPFEKLVYKFLKENLSD
LTFKQYEYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSI
ENLFEEKQNDTADILLVKDQFYELLDVKTRNISKSAQAPNIISAYKLAQT
CAKMIDNKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYIN
WAAAMQIQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVK
PFKKYI
3D structure
PDB2gie Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
ChainD
Resolution2.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D G30 H31 A33 Q109 N110 D111 D114 T130 N132 K135 S136 A137 Q138 P140 N141 S144 K147 A205 G29 H30 A32 Q108 N109 D110 D113 T129 N131 K134 S135 A136 Q137 P139 N140 S143 K146 A204 PDBbind-CN: Kd=0.58nM
BS02 dna D Y77 G92 K93 K108 Q109 Q138 Y199 N201 K248 Y76 G91 K92 K107 Q108 Q137 Y198 N200 K247 PDBbind-CN: Kd=0.58nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2gie, PDBe:2gie, PDBj:2gie
PDBsum2gie
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

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