Structure of PDB 2f2h Chain D

Receptor sequence
>2f2hD (length=773) Species: 562 (Escherichia coli) [Search protein sequence]
MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRERTWQLDT
PLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERY
AEFKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYM
FERLDLGVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFY
MTNRGYGVLVNHPQCVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAV
LDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPL
HVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKICVWINPYI
GQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYAD
KLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSI
GLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWA
YDDESCDVVRFFTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPA
CDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEGRWTHLWHNDELDGSRWH
KQQHGFLSLPVYVRDNTLLALGNNDQRPDYVWHEGTAFHLFNLQDGHEAV
CEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQDG
SQAESEQGLVVKPQGNALTITLH
3D structure
PDB2f2h Expanding the Thioglycoligase Strategy to the Synthesis of alpha-linked Thioglycosides Allows Structural Investigation of the Parent Enzyme/Substrate Complex
ChainD
Resolution1.95 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.2.1.177: alpha-D-xyloside xylohydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 XTG D D185 D306 C307 K414 D416 R466 W479 D482 F515 H540 D185 D306 C307 K414 D416 R466 W479 D482 F515 H540 MOAD: Ki=2uM
PDBbind-CN: -logKd/Ki=5.70,Ki=2uM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0030246 carbohydrate binding
GO:0042802 identical protein binding
GO:0061634 alpha-D-xyloside xylohydrolase
GO:0080176 xyloglucan 1,6-alpha-xylosidase activity
Biological Process
GO:0005975 carbohydrate metabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:2f2h, PDBe:2f2h, PDBj:2f2h
PDBsum2f2h
PubMed16478160
UniProtP31434|XYLS_ECOLI Alpha-xylosidase (Gene Name=yicI)

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