Structure of PDB 2cnb Chain D

Receptor sequence

>2cnbD (length=364) Species: 5691 (Trypanosoma brucei)

SHMRVLVCGGAGYIGSHFVRALLRDTNHSVVIVDSLVGTHGKSDHVETRE
NVARKLQQSDGPKPPWADRYAALEVGDVRNEDFLNGVFTRHGPIDAVVHM
CAFLAVGESVRDPLKYYDNNVVGILRLLQAMLLHKCDKIIFSSSAAIFGN
PTNAEPIDINAKKSPESPYGESKLIAERMIRDCAEAYGIKGICLRYFNAC
GAHEDGDIGEHYQGSTHLIPIILGRVMSDIADKRMPIFGTDYPTPDGTCV
RDYVHVCDLASAHILALDYVEKLGPNDKSKYFSVFNLGTSRGYSVREVIE
VARKTTGHPIPVRECGRREGDPAYLVAASDKAREVLGWKPKYDTLEAIME
TSWKFQRTHPNGYA

3D structure

• PDB: 2cnb Trypanosoma Brucei Udp-Galactose-4-Epimerase in Ternary Complex with Nad+ and the Substrate Analogue Udp-4-Deoxy-4-Fluoro-Alpha-D-Galactose
• Chain: D
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S142 A143 A144 Y173 K177
• Catalytic site (residue number reindexed from 1): S144 A145 A146 Y169 K173
• Enzyme Commision number: 5.1.3.2: UDP-glucose 4-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UFG	D	L102 V104 S142 Y173 F201 N202 H221 L222 I225 M252 I254 F255 C266 R268 V312 R335 D338	L104 V106 S144 Y169 F197 N198 H217 L218 I221 M235 I237 F238 C249 R251 V295 R318 D321
BS02	NAD	D	G7 G10 Y11 I12 D32 S33 L34 V35 G36 D75 M98 C99 A100 L102 N117 S140 S142 Y173 K177 Y200 F201 A203	G9 G12 Y13 I14 D34 S35 L36 V37 G38 D77 M100 C101 A102 L104 N119 S142 S144 Y169 K173 Y196 F197 A199

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003978 UDP-glucose 4-epimerase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0006012 galactose metabolic process
• GO:0033499 galactose catabolic process via UDP-galactose

Cellular Component

• GO:0005829 cytosol
• GO:0020015 glycosome

External links

• PDB: RCSB:2cnb, PDBe:2cnb, PDBj:2cnb
• PDBsum: 2cnb
• PubMed: 16946458
• UniProt: Q8T8E9