Structure of PDB 1v15 Chain D

Receptor sequence

>1v15D (length=133) Species: 562 (Escherichia coli)

ESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFD
DFRKAVWEEVSKDPELSKNLNPSNKSSVSKGYSPFTPKNQQVGGRKVYEL
HADKPISQGGEVYDMDNIRVTTPKRHIDIHRGK

3D structure

• PDB: 1v15 Structure-Based Analysis of the Metal-Dependent Mechanism of H-N-H Endonucleases
• Chain: D
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R5 R96 E100 H102 A103 H127 H131
• Catalytic site (residue number reindexed from 1): R4 R95 E99 H101 A102 H126 H130
• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	S74 S78 K81 Y83	S73 S77 K80 Y82
BS02	dna	D	P73 S74 S77	P72 S73 S76
BS03	dna	D	L23 D24 N70 L71 N72 N75 F86 T87 P88 K89 Q92 Y99	L22 D23 N69 L70 N71 N74 F85 T86 P87 K88 Q91 Y98
BS04	dna	D	R5 D51 R54 Y83 S84 V98 E100 L101 H102 S108 H127 H131	R4 D50 R53 Y82 S83 V97 E99 L100 H101 S107 H126 H130
BS05	dna	D	V93 G94 G95 R96 R132	V92 G93 G94 R95 R131
BS06	ZN	D	H102 H127 H131	H101 H126 H130

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity
• GO:0005102 signaling receptor binding

Biological Process

• GO:0009617 response to bacterium
• GO:0019835 cytolysis

External links

• PDB: RCSB:1v15, PDBe:1v15, PDBj:1v15
• PDBsum: 1v15
• PubMed: 15190054
• UniProt: P09883|CEA9_ECOLX Colicin-E9 (Gene Name=col)