Structure of PDB 1tt5 Chain D

Receptor sequence
>1tt5D (length=395) Species: 9606 (Homo sapiens) [Search protein sequence]
WNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKN
LALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVP
NCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYED
GVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMC
TIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLE
RASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYI
PLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQLQEVLDYLTNSA
SLQMKSPAITATKNRTLYLQVTSIEERTRPLAVADVTTPQTVLFK
3D structure
PDB1tt5 A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
ChainD
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K21 D146 C216 T217 A219
Catalytic site (residue number reindexed from 1) K5 D130 C200 T201 A203
Enzyme Commision number 6.2.1.64: E1 NEDD8-activating enzyme.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 peptide D H32 F44 R136 Q137 F138 H139 P176 L193 I310 H16 F28 R120 Q121 F122 H123 P160 L177 I294
BS02 ZN D C199 C202 C343 C346 C183 C186 C327 C330
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008641 ubiquitin-like modifier activating enzyme activity
GO:0016874 ligase activity
GO:0019781 NEDD8 activating enzyme activity
GO:0019788 NEDD8 transferase activity
GO:0042802 identical protein binding
GO:0046982 protein heterodimerization activity
Biological Process
GO:0000278 mitotic cell cycle
GO:0006508 proteolysis
GO:0007113 endomitotic cell cycle
GO:0036211 protein modification process
GO:0043687 post-translational protein modification
GO:0045116 protein neddylation
GO:0051726 regulation of cell cycle
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1tt5, PDBe:1tt5, PDBj:1tt5
PDBsum1tt5
PubMed15361859
UniProtQ8TBC4|UBA3_HUMAN NEDD8-activating enzyme E1 catalytic subunit (Gene Name=UBA3)

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