Structure of PDB 1pjl Chain D

Receptor sequence

>1pjlD (length=551) Species: 9606 (Homo sapiens) [Search protein sequence]

EKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNL
KKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQ
YGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDL
GVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLY
QKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREK
YCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANL
IVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPES
IPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNP
TAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFP
GVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEV
SINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEW
P

3D structure

PDB

1pjl Potent and competitive inhibition of malic enzymes by lanthanide ions

Chain

Resolution

2.9 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y3112 R3165 K3183 E3255 D3256 D3278 D3279 N3421
Catalytic site (residue number reindexed from 1)	Y90 R143 K161 E233 D234 D256 D257 N399
Enzyme Commision number	1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	D	R3165 T3283 L3310 G3311 A3312 G3313 E3314 A3315 D3345 K3346 V3392 A3393 A3395 L3398 L3419 S3420 N3421 G3465 N3467	R143 T261 L288 G289 A290 G291 E292 A293 D323 K324 V370 A371 A373 L376 L397 S398 N399 G443 N445
BS02	NAD	D	H3154 K3156 I3193 R3194 L3480 R3542 R3556	H132 K134 I171 R172 L458 R520 R534

Gene Ontology

	Molecular Function
GO:0004470	malic enzyme activity
GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948	oxaloacetate decarboxylase activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872	metal ion binding
GO:0051287	NAD binding

	Biological Process
GO:0006090	pyruvate metabolic process
GO:0006108	malate metabolic process
GO:1902031	regulation of NADP metabolic process

	Cellular Component
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0043231	intracellular membrane-bounded organelle

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1pjl, PDBe:1pjl, PDBj:1pjl
PDBsum	1pjl
PubMed	10913357
UniProt	P23368\|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)

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