Structure of PDB 1msw Chain D

Receptor sequence

>1mswD (length=863) Species: 10760 (Escherichia phage T7) [Search protein sequence]

NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPT
AFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARF
GRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAW
SSWHKEDSIHVGVRCIEMLIESTGMVSLHRQSETIELAPEYAEAIATRAG
ALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYE
DVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREE
LPMKTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNM
DWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGV
DKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAG
VQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDI
YGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQW
LAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQP
NQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILR
KRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEID
AHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPAD
AANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNL
NLRDILESDFAFA

3D structure

PDB

1msw Structural Basis for the Transition from Initiation to Elongation Transcription in T7 RNA Polymerase

Chain

Resolution

2.1 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	D	R57 R298 H300 R357 K392 D421 W422 R423 Y427 M431 T636 Y639 F644 G645 Y739 H772 S776 P780	R56 R289 H291 R348 K372 D401 W402 R403 Y407 M411 T616 Y619 F624 G625 Y719 H752 S756 P760
BS02	dna	D	K164 K172 R173 Y178 W377 A382 Y385 Q656 D660 N671	K163 K171 R172 Y177 W357 A362 Y365 Q636 D640 N651
BS03	rna	D	N171 S301 K389 A390 S393 R394 R425 Y639 R756 I810 H811 D812	N170 S292 K369 A370 S373 R374 R405 Y619 R736 I790 H791 D792

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034062	5'-3' RNA polymerase activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0019083	viral transcription
GO:0039695	DNA-templated viral transcription

	Cellular Component
GO:0000428	DNA-directed RNA polymerase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1msw, PDBe:1msw, PDBj:1msw
PDBsum	1msw
PubMed	12242451
UniProt	P00573\|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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