Structure of PDB 1mio Chain D

Receptor sequence

>1mioD (length=457) Species: 1501 (Clostridium pasteurianum)

LDATPKEIVERKALRINPAKTCQPVGAMYAALGIHNCLPHSHGSQGCCSY
HRTVLSRHFKEPAMASTSSFTEGASVFGGGSNIKTAVKNIFSLYNPDIIA
VHTTCLSETLGDDLPTYISQMEDAGSIPEGKLVIHTNTPSYVGSHVTGFA
NMVQGIVNYLSENTGAKNGKINVIPGFVGPADMREIKRLFEAMDIPYIMF
PDTSGVLDGPTTGEYKMYPEGGTKIEDLKDTGNSDLTLSLGSYASDLGAK
TLEKKCKVPFKTLRTPIGVSATDEFIMALSEATGKEVPASIEEERGQLID
LMIDAQQYLQGKKVALLGDPDEIIALSKFIIELGAIPKYVVTGTPGMKFQ
KEIDAMLAEAGIEGSKVKVEGDFFDVHQWIKNEGVDLLISNTYGKFIARE
ENIPFVRFGFPIMDRYGHYYNPKVGYKGAIRLVEEITNVILDKIERECTE
EDFEVVR

3D structure

• PDB: 1mio X-ray crystal structure of the nitrogenase molybdenum-iron protein from Clostridium pasteurianum at 3.0-A resolution.
• Chain: D
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C106 T110
• Catalytic site (residue number reindexed from 1): C105 T109
• Enzyme Commision number: 1.18.6.1: nitrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	D	D301 D305	D300 D304
BS02	CA	D	K61 E62	K60 E61
BS03	CLP	D	C23 P25 S45 G47 C48 Y51 H52 T105 C106 S141	C22 P24 S44 G46 C47 Y50 H51 T104 C105 S140

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016163 nitrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding

Biological Process

• GO:0009399 nitrogen fixation

Cellular Component

• GO:0016612 molybdenum-iron nitrogenase complex

External links

• PDB: RCSB:1mio, PDBe:1mio, PDBj:1mio
• PDBsum: 1mio
• PubMed: 8393705
• UniProt: P11347|NIFK_CLOPA Nitrogenase molybdenum-iron protein beta chain (Gene Name=nifK)