Structure of PDB 1m54 Chain D

Receptor sequence

>1m54D (length=346) Species: 9606 (Homo sapiens)

DAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIG
KKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPT
SGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTESH
VGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVA
SVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEV
EGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGST
VAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKE

3D structure

• PDB: 1m54 HUMAN CYSTATHIONINE BETA-SYNTHASE IS A HEME SENSOR PROTEIN. EVIDENCE THAT THE REDOX SENSOR IS HEME AND NOT THE VICINAL CYSTEINES IN THE CXXC MOTIF SEEN IN THE CRYSTAL STRUCTURE OF THE TRUNCATED ENZYME
• Chain: D
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K119 S147 D281 S285 L287 S349 P375
• Catalytic site (residue number reindexed from 1): K73 S101 D228 S232 L234 S296 P322
• Enzyme Commision number: 4.2.1.22: cystathionine beta-synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	D	K119 N149 V255 G256 T257 G258 G259 T260 G305 I306 S349 P375	K73 N103 V202 G203 T204 G205 G206 T207 G252 I253 S296 P322
BS02	HEM	D	R51 C52 T53 W54 R58 E62 S63 P64 H65 A226 P229 L230 R266	R5 C6 T7 W8 R12 E16 S17 P18 H19 A173 P176 L177 R213

Gene Ontology

Molecular Function

• GO:0004122 cystathionine beta-synthase activity

Biological Process

• GO:0006535 cysteine biosynthetic process from serine
• GO:0019343 cysteine biosynthetic process via cystathionine

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1m54, PDBe:1m54, PDBj:1m54
• PDBsum: 1m54
• PubMed: 12173932
• UniProt: P35520|CBS_HUMAN Cystathionine beta-synthase (Gene Name=CBS)