Structure of PDB 1ky5 Chain D

Receptor sequence

>1ky5D (length=430) Species: 10116 (Rattus norvegicus) [Search protein sequence]

DKLPYKVADIGLAAWGRKALDIAENEMPGLMRMREMYSASKPLKGARIAG
CLHMTVETAVLIETLVALGAEVRWSSCNIFSTQDHAAAAIAKAGIPVFAW
KGETDEEYLWCIEQTLHFKDGPLNMILDDGGDLTNLIHTKHPQLLSGIRG
ISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDG
IKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIEPINALQA
AMEGYEVTTMDEACKEGNIFVTTTGCVDIILGRHFEQMKDDAIVCNIGHF
DVEIDVKWLNENAVEKVNIKPQVDRYLLKNGHRIILLAEGRLVNLGCAMG
HPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKL
NVKLTKLTEKQAQYLGMPINGPFKPDHYRY

3D structure

PDB

1ky5 Catalytic Mechanism of S-adenosylhomocysteine hydrolase. Site-directed mutagenesis of Asp-130, Lys-185, Asp-189, and Asn-190.

Chain

Resolution

2.8 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H3054 S3077 S3082 D3130 E3155 N3180 K3185 D3189 N3190 C3194 H3300 H3352 S3360 Q3364
Catalytic site (residue number reindexed from 1)	H53 S76 S81 D129 E154 N179 K184 D188 N189 C193 H299 H351 S359 Q363
Enzyme Commision number	3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	NAI	D	K3425 Y3429	K424 Y428
BS02	NAI	D	T3156 T3157 T3158 N3190 G3219 G3221 V3223 T3241 E3242 I3243 T3274 T3275 I3280 I3298 G3299 H3300 L3343 N3345 H3352	T155 T156 T157 N189 G218 G220 V222 T240 E241 I242 T273 T274 I279 I297 G298 H299 L342 N344 H351
BS03	ADY	D	L3053 H3054 T3056 T3059 D3130 E3155 T3156 D3189 H3300 H3352 M3357	L52 H53 T55 T58 D129 E154 T155 D188 H299 H351 M356

Gene Ontology

	Molecular Function
GO:0004013	adenosylhomocysteinase activity
GO:0005507	copper ion binding
GO:0016787	hydrolase activity
GO:0030554	adenyl nucleotide binding
GO:0042802	identical protein binding
GO:0051287	NAD binding
GO:0098604	adenosylselenohomocysteinase activity

	Biological Process
GO:0001666	response to hypoxia
GO:0002439	chronic inflammatory response to antigenic stimulus
GO:0006730	one-carbon metabolic process
GO:0007584	response to nutrient
GO:0019510	S-adenosylhomocysteine catabolic process
GO:0033353	S-adenosylmethionine cycle
GO:0042745	circadian sleep/wake cycle

	Cellular Component
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005829	cytosol
GO:0042470	melanosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1ky5, PDBe:1ky5, PDBj:1ky5
PDBsum	1ky5
PubMed	11927587
UniProt	P10760\|SAHH_RAT Adenosylhomocysteinase (Gene Name=Ahcy)

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