Structure of PDB 1gz4 Chain D

Receptor sequence

>1gz4D (length=551) Species: 9606 (Homo sapiens) [Search protein sequence]

EKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNL
KKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQ
YGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDL
GVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLY
QKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREK
YCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANL
IVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPES
IPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNP
TAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFP
GVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEV
SINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEW
P

3D structure

PDB

1gz4 Molecular Mechanism for the Regulation of Human Mitochondrial Nad(P)(+)-Dependent Malic Enzyme by ATP and Fumarate

Chain

Resolution

2.2 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y112 R165 K183 E255 D256 D278 D279 N421
Catalytic site (residue number reindexed from 1)	Y90 R143 K161 E233 D234 D256 D257 N399
Enzyme Commision number	1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	D	R165 N259 G311 A312 G313 E314 A315 D345 K346 V392 A393 L398	R143 N237 G289 A290 G291 E292 A293 D323 K324 V370 A371 L376	MOAD: Ki=0.2mM
BS02	ATP	D	H154 I193 R194 R197 I479 L480 N482 R542 R556	H132 I171 R172 R175 I457 L458 N460 R520 R534	MOAD: Ki=0.2mM
BS03	TTN	D	Y112 R165 L167 D279 N421 N466 N467	Y90 R143 L145 D257 N399 N444 N445
BS04	MN	D	E255 D256 D279	E233 D234 D257
BS05	FUM	D	R67 R91 L95	R45 R69 L73

Gene Ontology

	Molecular Function
GO:0004470	malic enzyme activity
GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948	oxaloacetate decarboxylase activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872	metal ion binding
GO:0051287	NAD binding

	Biological Process
GO:0006090	pyruvate metabolic process
GO:0006108	malate metabolic process
GO:1902031	regulation of NADP metabolic process

	Cellular Component
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0043231	intracellular membrane-bounded organelle

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1gz4, PDBe:1gz4, PDBj:1gz4
PDBsum	1gz4
PubMed	12121650
UniProt	P23368\|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)

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