Structure of PDB 1evw Chain D

Receptor sequence
>1evwD (length=162) Species: 5791 (Physarum polycephalum) [Search protein sequence]
ALTNAQILAVIDSWEETVGQFPVITHHVPLGGGLQGTLHCYEIPLAAPYG
VGFAKNGPTRWQYKRTINQVVHRWGSHTVPFLLEPDNINGKTCTASHLCH
NTRCHNPLHLCWESADDNKGRNWCPGPNGGCVHAVVCLRQGPLYGPGATV
AGPQQRGSHFVV
3D structure
PDB1evw Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.
ChainD
Resolution3.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N119
Catalytic site (residue number reindexed from 1) N118
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna D N57 R61 Q63 R74 A96 S97 H98 N119 N56 R60 Q62 R73 A95 S96 H97 N118 PDBbind-CN: Kd=10pM
BS02 dna D P49 V52 G53 N57 K65 P48 V51 G52 N56 K64 PDBbind-CN: Kd=10pM
BS03 ZN D C125 C132 H134 C138 C124 C131 H133 C137
BS04 ZN D C41 C100 C105 H110 C40 C99 C104 H109
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
Biological Process
GO:0006314 intron homing

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Molecular Function
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Biological Process
External links
PDB RCSB:1evw, PDBe:1evw, PDBj:1evw
PDBsum1evw
PubMed10891275
UniProtQ94702|PPO1_PHYPO Intron-encoded endonuclease I-PpoI

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