Structure of PDB 1ecb Chain D

Receptor sequence
>1ecbD (length=473) Species: 562 (Escherichia coli) [Search protein sequence]
CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGL
VSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAH
NGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEAD
NIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDEN
RTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSN
PCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIP
IPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMSVRRKLNANRAEFR
DKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGID
MPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFEC
SVFNGVYVTKDVDQGYLDFLDTL
3D structure
PDB1ecb Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site.
ChainD
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C1 G27 N101 G102 Y258 R321 K326 M336 D444
Catalytic site (residue number reindexed from 1) C1 G27 N101 G102 Y258 R321 K326 M336 D436
Enzyme Commision number 2.4.2.14: amidophosphoribosyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 5GP D V325 K326 R328 V325 K326 R328
BS02 MG D D366 D367 D358 D359
BS03 5GP D Y74 Y258 D366 D367 V370 R371 G372 T374 Y74 Y258 D358 D359 V362 R363 G364 T366
BS04 5GP D H25 Y258 A260 R261 R275 P302 E303 T304 K326 H25 Y258 A260 R261 R275 P302 E303 T304 K326
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004044 amidophosphoribosyltransferase activity
GO:0016757 glycosyltransferase activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0097216 guanosine tetraphosphate binding
Biological Process
GO:0006164 purine nucleotide biosynthetic process
GO:0006189 'de novo' IMP biosynthetic process
GO:0006541 glutamine metabolic process
GO:0009113 purine nucleobase biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Biological Process
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Cellular Component
External links
PDB RCSB:1ecb, PDBe:1ecb, PDBj:1ecb
PDBsum1ecb
PubMed9333323
UniProtP0AG16|PUR1_ECOLI Amidophosphoribosyltransferase (Gene Name=purF)

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