Structure of PDB 8i9w Chain Cb

Receptor sequence

>8i9wCb (length=634) Species: 759272 (Thermochaetoides thermophila DSM 1495)

MPKRAVSPAPSENEVDIAGSLFAGFQAMGLDANLLRAIARKGFSVPTPIQ
RKTIPLILERRDVVGMARTGSGKTAAFVIPMIERLKAHSARFGARALVLS
PSRELALQTLKVVKELGKGTDLKTVLLVGGDSLEEQFGLMATNPDIIIAT
PGRFLHLKVEMSLDLSSIKYVVFDEADRLFEMGFAAQLTEILHALPPSRQ
TLLFSATLPSSLVEFTRAGLQDPVLVRLDAETKVSPDLESAFFSVKSGEK
EGALLHILYDIIKMPLGDPPKPTEHSTIIFTATKHHVEYISNLLRLAGFS
VSYVYGSLDQTARKIQVDNFRRGRTNILVVTDVAARGIDIPVLANVINYD
FPPQPKIFVHRVGRTARAGQRGWAYALVRESDLPYLLDLQLFLGRRLVLG
REEKDPSFARDIVVGSLKRVELENNVEWVNKVLHENEDIGALKRVTAKAE
KLYMKTRNPASSQSAKRAREVIVSKGWGQLHAIFGEEAANEEQVRDNLLS
KITGYKPQETIFEINKAAEAVRSFRQRIGPRKSFADPEVYMSYTPRVKMI
RGESGVKIAASFKSGRFEKWRQQHRLGRLPQVGEMEKANLVRNFSLPSGP
RFKHKQMKAPKEADKWRDDYEVRKKRVAEAKEKR

3D structure

• PDB: 8i9w Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
• Chain: Cb
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Cb	K3 R4 R107 P168 R170 E182 T217 H223 F251 K540 R622 G629 R631 G798 E799 S800 V802 I804 F808 S810 R812 K815 Q819 F848 H850 Q852 M853 K854 P856 W862 R863	K3 R4 R40 P101 R103 E115 T150 H156 F184 K448 R522 G529 R531 G552 E553 S554 V556 I558 F562 S564 R566 K569 Q573 F602 H604 Q606 M607 K608 P610 W616 R617

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:8i9w, PDBe:8i9w, PDBj:8i9w
• PDBsum: 8i9w
• PubMed: 37129998
• UniProt: G0S5S8