Structure of PDB 5mei Chain CT

Receptor sequence

>5meiCT (length=188) Species: 559292 (Saccharomyces cerevisiae S288C)

ANLRTQKRLAASVVGVGKRKVWLDPNETSEIAQANSRNAIRKLVKNGTIV
KKAVTVHSKSRTRAHAQSKREGRHSGYGKRKGTREARLPSQVVWIRRLRV
LRRLLAKYRDAGKIDKHLYHVLYKESKGNAFKHKRALVEHIIQAKADAQR
EKALNEEAEARRLKNRAARDRRAQRVAEKRDALLKEDA

3D structure

• PDB: 5mei Inhibition of Eukaryotic Translation by the Antitumor Natural Product Agelastatin A.
• Chain: CT
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	CT	R162 R163 K165 R170 R172 R173	R161 R162 K164 R169 R171 R172
BS02	rna	CT	A2 N3 L4 T6 K8 R9 G18 K19 R20 K21 W23 L24 P26 S37 R38 N39 R42 K43 K46 K53 T56 V57 H58 S59 K60 S61 R62 T63 R64 K70 R71 R74 H75 G77 Y78 G79 K80 R81 K82 G83 T84 R85 R88 L89 Q92 V93 W95 I96 R97 L99 R100 R103 R104 R110 K114 K117 H118 Y120 H121 Y124 K125 K128 G129 H134 K135 R136 I143	A1 N2 L3 T5 K7 R8 G17 K18 R19 K20 W22 L23 P25 S36 R37 N38 R41 K42 K45 K52 T55 V56 H57 S58 K59 S60 R61 T62 R63 K69 R70 R73 H74 G76 Y77 G78 K79 R80 K81 G82 T83 R84 R87 L88 Q91 V92 W94 I95 R96 L98 R99 R102 R103 R109 K113 K116 H117 Y119 H120 Y123 K124 K127 G128 H133 K134 R135 I142
BS03	OHX	CT	A87 R88	A86 R87

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5mei, PDBe:5mei, PDBj:5mei
• PDBsum: 5mei
• PubMed: 28457705
• UniProt: P0CX82|RL19A_YEAST Large ribosomal subunit protein eL19A (Gene Name=RPL19A)