Structure of PDB 6rxt Chain CI

Receptor sequence

>6rxtCI (length=822) Species: 209285 (Thermochaetoides thermophila)

ARQAARSHDIKEKRLHVPLVDRLPDEPPPRLVVIVGPPGVGKTTLLKSLV
RRYTKETMSDPVGPITVVTSKKQRLTFIECPNELEAMIDMAKVADIVLLM
IDGNYGFEMETMEFLNILANTGMPGNVFGILTHLDLFKKPSALKDAKKRL
KHRLWTELYQGAHLFYLSGVLNGRYPDREIHNLSRFLSVMKNPRPLVWRN
THPYTIIDNYRDITHPTKIEEDPLCDRTIELSGYLRGTNFAAQGQRVHIA
GVGDFTISKIEELPDPCPTPAMEKAPRRRLDEKDKKLWAPMADRSGMKIS
GDHIVITREKGFTFDKDANVERGEGEQLIVDLQGEKKLLGQTDKGVKLFA
GGEQLTQKPWRAIDLARLMYDTTLTPAQALRRWRGDYEELKTKWSNPENI
DALRRTRFQWYEMQKAMLQKQLDINKAEYAELDEHQRRQVEGYRAGKYAR
LVIEGVPAEFCKNFQPRMPILVGGLSATEDRFGFVQVRIKRHRWHKKILK
TGDPLIFSLGWRRFQTLPIYSIWDNRTRNRMLKYTPEHMHCFGTFWGPLI
APNTSFCCFQSFSASNPGFRIAATGTVLSVDESTEIVKKLKLVGTPWKIF
KNTAFIKDMFNSSLEIAKFEGAAIRTVSGIRGQIKRALSKPEGYFRATFE
DKILLSDIVILKAWYPVKPKQFYNPATNLIGWQSMRLTGEIRRAENIPTP
QNPNSTYRKIERPERHFNPLRVPKNLAAELPFKSQIVQTKPQKKETYMQK
RAVVVGREERKLRDLMQKLTTIRKEKIAKRKAKKEAQREKLKKELAEIEE
RRREKQKKEKKEFWEREGKKRK

3D structure

• PDB: 6rxt Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
• Chain: CI
• Resolution: 7.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	CI	R41 R45 K178 P179 S180 K183 K190 R217 H220 N221 I826 K828 N853 R856 R858 K861 Y862 E865 H866 M867 K916 K917 K919 W925 F928 K929 N930 T931 F933 R953 V955 R959 K963 R964 L966 S967 R974 E978 K980 L982 L983 I986 I988 K990 R1020 P1028 Q1029 S1033 Y1035 R1043 H1044 F1045 N1046 R1049 N1053 S1062 T1067 P1069 Q1070 K1071 K1072 T1074 Y1075 Q1077 R1085 R1088 R1091 D1092 Q1095 T1098 T1099 K1104 K1107 R1108 K1111 K1112 R1116 W1142 E1143 G1146 K1147	R2 R6 K139 P140 S141 K144 K151 R178 H181 N182 I498 K500 N525 R528 R530 K533 Y534 E537 H538 M539 K588 K589 K591 W597 F600 K601 N602 T603 F605 R625 V627 R631 K635 R636 L638 S639 R646 E650 K652 L654 L655 I658 I660 K662 R692 P700 Q701 S705 Y707 R715 H716 F717 N718 R721 N725 S734 T739 P741 Q742 K743 K744 T746 Y747 Q749 R757 R760 R763 D764 Q767 T770 T771 K776 K779 R780 K783 K784 R788 W814 E815 G818 K819
BS02	rna	CI	K1135 K1138 F1141 W1142	K807 K810 F813 W814
BS03	GTP	CI	G78 G80 K81 T82 T83 T171 H172 D174 L175 V209	G39 G41 K42 T43 T44 T132 H133 D135 L136 V170
BS04	MG	CI	T82 E118	T43 E79

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0034511 U3 snoRNA binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000479 endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030686 90S preribosome

External links

• PDB: RCSB:6rxt, PDBe:6rxt, PDBj:6rxt
• PDBsum: 6rxt
• PubMed: 31378463
• UniProt: G0S445