Structure of PDB 5j88 Chain CF

Receptor sequence

>5j88CF (length=177) Species: 83333 (Escherichia coli K-12)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

• PDB: 5j88 Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.
• Chain: CF
• Resolution: 3.32 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	CF	S24 M26 Q27 Q63 K64 L66 K69 K88 T90 R92 K161	S23 M25 Q26 Q62 K63 L65 K68 K87 T89 R91 K160
BS02	rna	CF	K33 N37 G39 G41 T68 A70 R71 K72 F77 I79 I85 K88 S121 D123 R125 S129 M130 G131 R133 G151 D153 T155	K32 N36 G38 G40 T67 A69 R70 K71 F76 I78 I84 K87 S120 D122 R124 S128 M129 G130 R132 G150 D152 T154

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5j88, PDBe:5j88, PDBj:5j88
• PDBsum: 5j88
• PubMed: 27382179
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)