Structure of PDB 5it8 Chain CF

Receptor sequence

>5it8CF (length=177) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

5it8 Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.

Chain

Resolution

3.12 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	CF	S24 V25 M26 Q27 Q63 K64 L66 K69 K88 T90 R92 K161	S23 V24 M25 Q26 Q62 K63 L65 K68 K87 T89 R91 K160
BS02	rna	CF	K33 T35 N37 G39 G41 T68 R71 K72 F77 I79 I85 K88 S121 D123 S129 G131 V132 R133 G151 D153 T155	K32 T34 N36 G38 G40 T67 R70 K71 F76 I78 I84 K87 S120 D122 S128 G130 V131 R132 G150 D152 T154

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5it8, PDBe:5it8, PDBj:5it8
PDBsum	5it8
PubMed	27382179
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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