Structure of PDB 8wx0 Chain C

Receptor sequence

>8wx0C (length=593) Species: 1773 (Mycobacterium tuberculosis)

IDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAVVAYLDDDNMLLSA
TTASKNPKEHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCR
LIDRPLRPSFVDGLRNEIQIVVTILSLDPGDLYDVLAINAASASTQLGGL
PFSGPIGGVRVALIDGTWVGFPTVDQIERAVFDMVVAGRIVEGDVAIMMV
EAEATENVVELVEGGAQAPTESVVAAGLEAAKPFIAALCTAQQELADAAG
KSGKPTVDFPVFPDYGEDVYYSVSSVATDELAAALTIGGKAERDQRIDEI
KTQVVQRLADTYEGREKEVGAALRALTKKLVRQRILTDHFRIDGRGITDI
RALSAEVAVVPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGPETSK
RYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYA
IRQVSEALGSNGSTSMGSVCASTLALLNAGVPLKAPVAGIAMGLVSDDIQ
VEGAVDGVVERRFVTLTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLD
GIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVT

3D structure

• PDB: 8wx0 Cryo-EM structures of Mycobacterium tuberculosis polynucleotide phosphorylase suggest a potential mechanism for its RNA substrate degradation.
• Chain: C
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	G87 F89 D397 S426	G82 F84 D392 S421
BS02	rna	C	F68 T72 R105 R112 R430	F63 T67 R100 R107 R425

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004654 polyribonucleotide nucleotidyltransferase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0006402 mRNA catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8wx0, PDBe:8wx0, PDBj:8wx0
• PDBsum: 8wx0
• PubMed: 38395123
• UniProt: P9WI57|PNP_MYCTU Polyribonucleotide nucleotidyltransferase (Gene Name=pnp)