Structure of PDB 8wwv Chain C

Receptor sequence

>8wwvC (length=490) Species: 1615674 (Pseudomonas lactis)

DFITKNNLWTNEQRDAADKVLAEIDSLGLEMIRLSWADQYGLLRGKSLTV
ASLKSAFKEGSEVAIGPFFFDLVSSMVFNLFTTAGDFEDELSGNPTVVMV
PDPTTFKVLPWADKTGWMLADLHWKSGEPFPLCPRGIMKKAVKSLSDEGY
LFKCGIELEWYLTKIVDRSLSPESLGAPGVQPDAIQVQPVAQGYSVLLEH
HLDQVDDIMSKVRKGLLELNLPLRSIEDEWAPSQMETTFDVMEGLEAADA
ALLIKSAIKQICSRHGYHATFMCKPAINGFFASGWHMHQSLVDKDTRKNL
FIPSEGEVLSPLGRAYAGGLLANGSAASSFTTPTVNGYRRRQPYSLAPDR
RAWAKDNKAAMVRVVSATGDPASRIENRIGEPGANPYLYMASQIVSGLDG
IKNKKDPGELQESPYDAQVPMLPTTLAEALDALEHDSELFRSCFGDTFIK
YWLQLRRSEWARFLDAEGAEAAEPTGAVTQWEQKEYFNLL

3D structure

• PDB: 8wwv Discovery of the 1-naphthylamine biodegradation pathway reveals a glutamine synthetase-like protein that catalyzes 1-naphthylamine glutamylation
• Chain: C
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	C	G160 E162 R229 F244 D245 V246 H293 S295 K363 R368 R379	G155 E157 R224 F239 D240 V241 H288 S290 K358 R363 R374
BS02	P3S	C	E162 E164 E234 E241 G289 H291 R345 K363 R383	E157 E159 E229 E236 G284 H286 R340 K358 R378
BS03	MN	C	E162 H291 E381 R383	E157 H286 E376 R378
BS04	MN	C	E164 E234 E241	E159 E229 E236
BS05	MG	C	E162 E241	E157 E236

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane

External links

• PDB: RCSB:8wwv, PDBe:8wwv, PDBj:8wwv
• PDBsum: 8wwv
• UniProt: A0A7Y1Q2L1