Structure of PDB 8wwu Chain C

Receptor sequence

>8wwuC (length=491) Species: 1615674 (Pseudomonas lactis)

FDFITKNNLWTNEQRDAADKVLAEIDSLGLEMIRLSWADQYGLLRGKSLT
VASLKSAFKEGSEVAIGPFFFDLVSSMVFNLFTTAGDFEDELSGNPTVVM
VPDPTTFKVLPWADKTGWMLADLHWKSGEPFPLCPRGIMKKAVKSLSDEG
YLFKCGIELEWYLTKIVDRSLSPESLGAPGVQPDAIQVQPVAQGYSVLLE
HHLDQVDDIMSKVRKGLLELNLPLRSIEDEWAPSQMETTFDVMEGLEAAD
AALLIKSAIKQICSRHGYHATFMCKPAINGFFASGWHMHQSLVDKDTRKN
LFIPSEGEVLSPLGRAYAGGLLANGSAASSFTTPTVNGYRRRQPYSLAPD
RRAWAKDNKAAMVRVVSATGDPASRIENRIGEPGANPYLYMASQIVSGLD
GIKNKKDPGELQESPYDAQVPMLPTTLAEALDALEHDSELFRSCFGDTFI
KYWLQLRRSEWARFLDAEGAEAAEPTGAVTQWEQKEYFNLL

3D structure

• PDB: 8wwu Discovery of the 1-naphthylamine biodegradation pathway reveals a glutamine synthetase-like protein that catalyzes 1-naphthylamine glutamylation
• Chain: C
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	C	G160 E162 R229 F244 V246 H293 S295 K363 R368 R379 E381	G156 E158 R225 F240 V242 H289 S291 K359 R364 R375 E377
BS02	MN	C	E164 E234 E241	E160 E230 E237
BS03	MN	C	E162 H291 E381	E158 H287 E377
BS04	MG	C	E162 E241	E158 E237

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane

External links

• PDB: RCSB:8wwu, PDBe:8wwu, PDBj:8wwu
• PDBsum: 8wwu
• UniProt: A0A7Y1Q2L1