Structure of PDB 8uy2 Chain C

Receptor sequence

>8uy2C (length=553) Species: 759272 (Thermochaetoides thermophila DSM 1495)

AMHIRDMLAEAERTGEPSFSFEYFPPKTAQGVQNLYDRMERMYNYGPKFI
DITWGAGGRVAELTCEMVVQAQAYLGLETCMHLTCTDMGVERINDALRKA
YKAGCTNILALRGDPPRDKEKWEAAKDGFRYAKDLVAHIRKEYGDHFDIG
VAGYPEGCDDNKDEDLLLDHLKEKVDMGAGFIVTQMFYDVDNFLRWVKKV
RERGISVPIVPGIMPIATYASFLRRANHMKCKIPEEWMAKLEPVKNDDVA
VREIGKTLVADMCRKILDAGIRHLHFYTMNLAQATRMVLEELNWLPQDWD
EFPNGRWGDSRSPAFGELDAYGVGLTGSNEQNRERWGEPKCIRDIANLFI
RYLRKEIDYLPWSEAPVADEADLIKDELIDLNRRGLITVNSQPAVNGAKS
NHPVHGWGPSNGYVYQKAYLEFFVSPELYPEIKRRIESHPDLTYHAVTKS
GNLETNAQSDGPNAVTWGVFPGKEIVQPTIVERISFLAWKDEAYHLGMEW
ARCYDAGSPSRVLLEEMMNTWWLVNIVNNDFHQGNTLFEILKGLEVTDLD
KVP

3D structure

• PDB: 8uy2 Structural basis of S-adenosylmethionine-dependent allosteric transition from active to inactive states in methylenetetrahydrofolate reductase.
• Chain: C
• Resolution: 2.83 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	C	T52 W53 H81 T83 L110 R111 G112 D113 Y130 A131 A151 Y153 N160 K161 H169 E172 K173 V182 Q184 Y361	T53 W54 H82 T84 L111 R112 G113 D114 Y131 A132 A152 Y154 N161 K162 H170 E173 K174 V183 Q185 Y321
BS02	SAM	C	N344 Y392 A408 E410 T428 V429 N430 S431 Q432 Y459 T506 T519	N304 Y352 A368 E370 T388 V389 N390 S391 Q392 Y419 T466 T479
BS03	SAM	C	D246 D247 N344 E410 W447 V516 P518	D247 D248 N304 E370 W407 V476 P478

Gene Ontology

Molecular Function

• GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity
• GO:0016491 oxidoreductase activity
• GO:0071949 FAD binding

Biological Process

• GO:0009086 methionine biosynthetic process
• GO:0035999 tetrahydrofolate interconversion

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8uy2, PDBe:8uy2, PDBj:8uy2
• PDBsum: 8uy2
• PubMed: 38886362
• UniProt: G0S5U9