Structure of PDB 8sri Chain C

Receptor sequence

>8sriC (length=1368) Species: 946362 (Salpingoeca rosetta)

VAAKTLLIENEDGKGSTRMEVQDFMKRFHMHASEDDKTGSPSTAWGTLRF
PTKEATAPYLRLSVNDDPEDALLFVKAMLAQKYGETYDRPSLILSVTGGA
RNFTLPPRLETAIAKGLRLAAQRTNAWVVTGGTNTGVMKLTGQIMEALSK
TQSHFIPPTIGIATYGVIIGGDDMTRGEPPKIGLEYEMHKKDPPKTTPLD
DNHNLFLLVDDGSTNKFGKEIKFRAAFENAAGQAFAAPVVTIVVQGGPGT
LGTALQAVRQGTPIVVVDGSGLAADVLAYAYNFMHNPLTRFKSYTIDDLR
QKVAQTFNPKSSQQLTNLLDSALECVQDPNLVVVYSLQESGIDEFDDCIL
KAIFSSQGKLGNKLKQAMYFDQLDVAKRALSEASKNGQHNEIAACINDNL
MAAMMHNKPHFVELYLGFDAKIYELKPSEEVAKTNITALDELPSFALAIE
ELYKREAKKPHSHVQRLVSLSNTDVLGRHYRGRDLANTRAYNVLRMDQIF
ARLVSKDFSVNRDFTIYDSKYDKVPGIQFRRTAQASHMLFLWAICLDRFR
MARHFWLIGDQSIINALVASRILERLSTHRALQGPHLAEERAKMQHNAKK
FEELAVGVLGECHGSDSHMASEMLHSKNDMFNKKNAINIAYDAKSLAFLS
HPATQSVINADWYGHLKSVTSFWAVLFAFFFPFFVLPFINFSGAHRLRRK
FAKFYSAPYTRFISDLLSHFVLCVVTSYFVLDKLEDTISAIEWILLVWFV
ALLLEELRQMIFCDGIAEYISDTWNRLDLIMITLFFVGFFTHASDPSNQD
SKVVSKGIHAFLVVVLWLRFMRYYALSKNLGPKLIMMMEMMKDVSTFVFL
LLIFLIGYGVAAQSLLSPDEDFSSRTFIGVLFRPYFQIYGELFLDDLNSE
ANCLGDTPFTECSRETVRMVPFFLAVYILGSNVLLVNLLIAMFNDTYMKV
QEAAEDLWRKQNYELCAEYKDRPFLPAPFILLAHVHMLFMRLLRLCGVHT
QEHEKIQDDETKRKITTFEALNTDKFLRRWERERQEMLEARVKMTNDNVV
QAMGMMDQLLEHMISFRFSLDQQATKNRLNSAVAVHGHTAEAAEWYVPPE
EYPKSGGVKRYLIDASMVPLSIMCPSYDPVEYTHPSVAAQPVWADPADPR
KIKFNVKDEVNGKVVDRTSCHPSGISIDSNTGRPINPWGRTGMTGRGLLG
KWGVNQAADTVVTRWKRSPDGSILERDGKKVLEFVAIQRQDNKMWAIPGG
FVDNGEDVALTSGREFMEEALGMGTSAESKDSLAALFSSGTIVARIYCED
PRNTDNAWVETTCVNFHDESGRHAARLKLQGGDDAEHARWMMVHGGLNLF
ASHRTLLQHVTSALNAYF

3D structure

• PDB: 8sri Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution
• Chain: C
• Resolution: 2.73 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CLR	C	V942 L945 L946 L949 I972 L975 F976 Y979	V848 L851 L852 L855 I878 L881 F882 Y885
BS02	APR	C	T148 G149 G150 A151 R152 T184 F268 R275 G298 G300 T301	T97 G98 G99 A100 R101 T133 F217 R224 G247 G249 T250
BS03	MG	C	E850 Q853	E756 Q759
BS04	MG	C	G1370 E1390	G1249 E1269
BS05	MG	C	E1386 E1390 D1460	E1265 E1269 D1334
BS06	APR	C	W1264 D1330 R1360 G1370 G1371 F1372 E1390 D1426 R1428 F1476	W1143 D1209 R1239 G1249 G1250 F1251 E1269 D1300 R1302 F1350
BS07	CLR	C	F1003 R1012 P1015	F909 R918 P921
BS08	CLR	C	E1009 M1013 F1016	E915 M919 F922

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005262 calcium channel activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8sri, PDBe:8sri, PDBj:8sri
• PDBsum: 8sri
• UniProt: F2UB89