Structure of PDB 8sdb Chain C

Receptor sequence

>8sdbC (length=604) Species: 562 (Escherichia coli)

GTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHP
MRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPE
TASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSY
RELADQLVPYAKWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTR
DDFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSDWNTL
IYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEGEWI
PNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMG
GLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPL
SHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFA
QGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDP
YGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPG
KWREILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWL
VREA

3D structure

• PDB: 8sdb The Structure of Maltooctaose-Bound Escherichia coli Branching Enzyme Suggests a Mechanism for Donor Chain Specificity.
• Chain: C
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	C	E677 S683	E554 S560
BS02	GLC	C	N690 G694	N567 G571
BS03	GLC	C	D537 K546 G575	D414 K423 G452
BS04	GLC	C	D542 Q545	D419 Q422

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0043169 cation binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

External links

• PDB: RCSB:8sdb, PDBe:8sdb, PDBj:8sdb
• PDBsum: 8sdb
• PubMed: 37298853
• UniProt: P07762|GLGB_ECOLI 1,4-alpha-glucan branching enzyme GlgB (Gene Name=glgB)