Structure of PDB 8qhc Chain C

Receptor sequence

>8qhcC (length=393) Species: 866768 (Escherichia coli 'BL21-Gold(DE3)pLysS AG')

SKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQID
NAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDG
AILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIP
EPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKET
QKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMV
MPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS

3D structure

• PDB: 8qhc Structural basis for the toxicity of Legionella pneumophila effector SidH
• Chain: C
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	E55 R59 Y88 K90 N91 S220 I221 V227 E260 R263 G276 R284 R289 R319 H320 T321 G326 Y327 R328 Q330 T339 I364 E379 G380	E54 R58 Y87 K89 N90 S219 I220 V226 E259 R262 G275 R283 R288 R318 H319 T320 G325 Y326 R327 Q329 T338 I363 E378 G379
BS02	MG	C	T26 T62	T25 T61
BS03	GTP	C	D22 G24 K25 T26 T27 F47 T62 N136 K137 M140 S174 L176	D21 G23 K24 T25 T26 F46 T61 N135 K136 M139 S173 L175

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0032045 guanyl-nucleotide exchange factor complex

External links

• PDB: RCSB:8qhc, PDBe:8qhc, PDBj:8qhc
• PDBsum: 8qhc
• PubMed: 37923743
• UniProt: P0CE48|EFTU2_ECOLI Elongation factor Tu 2 (Gene Name=tufB)