Structure of PDB 8g0s Chain C

Receptor sequence

>8g0sC (length=648) Species: 5207 (Cryptococcus neoformans)

PLPDSVPESEDLFAPPPRMQGKEGRPKPHIGPNYESYVKEWAKTVGPNSD
EWWAAKARETLDWYDDFKTVRAGGFEHGDVQWFPEGTLNAAYNCLDRHYY
KNPKKTAIIYEADEPSESREVSYEELMQETCRVANVLKSYGVKKGDAVSI
YLPMTWQAAAAFLACARIGAIHSAVFAGFSAESLRDRVNDCECKVLITTD
EGRRGGKTIATKQIVDAALQQCPLVENVLVLRRTGNKVPMTEGRDKWWDE
ECAKMPAYCPCERMASEDPLFILYTSKPKGVVHSTAGYLLGTALTLKYVF
DAHPDDRFACMADIGWITGHSYIIYGPLANGITTAVFESTPVYPTPSRYW
DFVDKWKATQLYTAPTAIRLLRRMGEDHVKNHDLSSLRVLGSVGEPINPE
AWHWYNDFAGKNQCAIVDTYWMTETGSISIAPLPGAISTKPGSATFPFFG
MDVDIIDPQTGQVLEGNDVEGVLVARRPWPSIARTVYRDHKRYLETYMKP
YPGYFFFGDGAARDYDGYMWIKGRVDDVINVSGHRLSTAEVESALILHKG
VAETAVVGCADDLTGQAVYAFVTMKPEFDLKATKEADLSKELAIQVRKVI
GPFAAPKKIYLVSDLPKTRKIMRRVLRKIVAGEGDQPQIVEEVKQKVT

3D structure

• PDB: 8g0s Crystal Structure of Acetyl-CoA synthetase in complex with a cyclopentyl ester AMP inhibitor from Cryptococcus neoformans H99 (tetragonal form)
• Chain: C
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 6.2.1.1: acetate--CoA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	YHF	C	I335 T336 G412 E413 P414 T437 Y438 W439 M440 T441 D527 I539	I317 T318 G394 E395 P396 T419 Y420 W421 M422 T423 D509 I521

Gene Ontology

Molecular Function

• GO:0003987 acetate-CoA ligase activity
• GO:0005524 ATP binding
• GO:0016208 AMP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006085 acetyl-CoA biosynthetic process
• GO:0019427 acetyl-CoA biosynthetic process from acetate

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8g0s, PDBe:8g0s, PDBj:8g0s
• PDBsum: 8g0s
• UniProt: J9VFT1