Structure of PDB 8e82 Chain C

Receptor sequence
>8e82C (length=1095) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
SSSNNSVPGAPNRVSFAKLREPLEVPGLLDVQTDSFEWLIGSPRWRESAA
ERGDVNPVGGLEEVLYELSPIEDFSGSMSLSFSDPRFDDVKAPVDECKDK
DMTYAAPLFVTAEFINNNTGEIKSQTVFMGDFPMMTEKGTFIINGTERVV
VSQLVRSPGVYFDETIDKSTDKTLHSVKVIPSRGAWLEFDVDKRDTVGVR
IDRKRRQPVTVLLKALGWTSEQIVERFGFSEIMRSTLEKDNTVGTDEALL
DIYRKLRPGEPPTKESAQTLLENLFFKEKRYDLARVGRYKVNKKLGLHVG
EPITSSTLTEEDVVATIEYLVRLHEGQTTMTVPGGVEVPVETDDIDHFGN
RRLRTVGELIQNQIRVGMSRMERVVRERMTTQDVEAITPQTLINIRPVVA
AIKEFFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDV
HPSHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKVVDGVVS
DEIVYLTADEEDRHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSE
VDYMDVSPRQMVSVATAMIPFLEHDDANRALMGANMQRQAVPLVRSEAPL
VGTGMELRAAIDAGDVVVAEESGVIEEVSADYITVMHDNGTRRTYRMRKF
ARSNHGTCANQCPIVDAGDRVEAGQVIADGPCTDDGEMALGKNLLVAIMP
WEGHNYEDAIILSNRLVEEDVLTSIHIEEHEIDARDTKLGAEEITRDIPN
ISDEVLADLDERGIVRIGAEVRDGDILVGKVTPKGREVRDTSLKVPHGES
GKVIGIRVFSREDEDELPAGVNELVRVYVAQKRKISDGDKLAGRHGNKGV
IGKILPVEDMPFLADGTPVDIILNTHGVPRRMNIGQILETHLGWCAHSGW
KVDAAKGVPDWAARLPDELLEAQPNAIVSTPVFDGAQEAELQGLLSCTLP
NRDGDVLVDADGKAMLFDGRSGEPFPYPVTVGYMYIMKLHHLVDDKIHAR
STGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAYTLQELLTIKSD
DTVGRVKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLS
3D structure
PDB8e82 Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis.
ChainC
Resolution3.03 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna C N169 R173 R230 R421 K428 G1059 K1060 R1067 N144 R148 R205 R396 K403 G1015 K1016 R1023
BS02 dna C R181 W211 I370 L463 R467 V472 R156 W186 I345 L438 R442 V447
BS03 rna C Q435 R465 P489 Q614 K884 K892 H1035 M1051 L1057 Q1062 Q410 R440 P464 Q589 K840 K848 H991 M1007 L1013 Q1018
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0046677 response to antibiotic
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009274 peptidoglycan-based cell wall

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8e82, PDBe:8e82, PDBj:8e82
PDBsum8e82
PubMed37116494
UniProtP9WGY9|RPOB_MYCTU DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

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