Structure of PDB 8cfs Chain C

Receptor sequence

>8cfsC (length=460) Species: 208964 (Pseudomonas aeruginosa PAO1)

FTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILGC
IHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAWK
GETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLERIH
GITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSLND
AIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICAMQ
ACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDANML
KALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFDA
HNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYADL
PAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVSVE
GPFKPDTYRY

3D structure

• PDB: 8cfs Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry G11
• Chain: C
• Resolution: 1.64 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	K463 Y467	K454 Y458
BS02	UI7	C	D190 V422	D181 V413
BS03	NAD	C	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 I321 G322 H323 N375 H382	T156 T157 T158 N190 I218 G221 D222 V223 E242 V243 T289 N291 I312 G313 H314 N366 H373
BS04	ADE	C	H61 T63 Q65 L376 H382 M387	H52 T54 Q56 L367 H373 M378
BS05	UI7	C	T466 Y467	T457 Y458

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfs, PDBe:8cfs, PDBj:8cfs
• PDBsum: 8cfs
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)