Structure of PDB 8cfh Chain C

Receptor sequence

>8cfhC (length=462) Species: 208964 (Pseudomonas aeruginosa PAO1)

AGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKIL
GCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFA
WKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLER
IHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSL
NDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICA
MQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDAN
MLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGF
DAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYA
DLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVS
VEGPFKPDTYRY

3D structure

• PDB: 8cfh Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry C08
• Chain: C
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	K463 Y467	K456 Y460
BS02	R8V	C	N187 D190	N180 D183
BS03	R8V	C	D465 T466 R468	D458 T459 R461
BS04	NAD	C	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 I321 G322 H323 N375 H382	T158 T159 T160 N192 I220 G223 D224 V225 E244 V245 T291 N293 I314 G315 H316 N368 H375
BS05	ADE	C	H61 T63 H382 M387	H54 T56 H375 M380
BS06	R8V	C	T466 Y467 R468	T459 Y460 R461

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfh, PDBe:8cfh, PDBj:8cfh
• PDBsum: 8cfh
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)