Structure of PDB 8cfc Chain C

Receptor sequence

>8cfcC (length=463) Species: 208964 (Pseudomonas aeruginosa PAO1)

PAGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKI
LGCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVF
AWKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLE
RIHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHS
LNDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPIC
AMQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDA
NMLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDG
FDAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKY
ADLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGV
SVEGPFKPDTYRY

3D structure

• PDB: 8cfc Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry A01
• Chain: C
• Resolution: 1.64 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	K463 Y467	K457 Y461
BS02	NAD	C	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 I321 G322 H323 N375 H382	T159 T160 T161 N193 I221 G224 D225 V226 E245 V246 T292 N294 I315 G316 H317 N369 H376
BS03	ADE	C	H61 T63 Q65 L376 H382 M387	H55 T57 Q59 L370 H376 M381

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfc, PDBe:8cfc, PDBj:8cfc
• PDBsum: 8cfc
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)