Structure of PDB 8bx8 Chain C

Receptor sequence

>8bx8C (length=3947) Species: 5911 (Tetrahymena thermophila)

PLVWTQLKQTGTTQPTARSGHTIITVGKTHIMFGGLDNDKNNYKDGKIAP
NNQVFTLKLTQNNCEWRQIACQGDVPLPRCYHASCAISADKMLVFGGSYT
SNLRFNDTYILKTTSYQWSKPANQISGGEPKNAESKIGAPQPRYGHSATF
FEGKVYIFGGHGGINYQRLAFNDLYVLETENFEWTRLEPKGNPPDPRGGH
SAAMMANKPQLMIFGGWSFTSQYSNIMIYDIEKDEWVDPEIAHEIPKWNL
SGIMAPSIPSWKYFIFGGSVGSFEEGGNRTNSRFVDDSFVLDIDTLSWSS
INLEADETSKAVCKPRPRESASIFYDSGESRAIVFGGWANNWLNDLWALN
VSTITGPPIFSIKPALGPLTGKTKVLIEGDGFKDTQNISVKFSGGKLEKE
VNGTFVNEKEISCETPTFDYPRSVEVTVCMNKGDYTITKSAFTYNTKADK
TIAYGPGLLTENLIGVQTTIVIQARNGSDEFVVTIRNPAKIKKEEEVKEG
DKANTKNTIKEDEEEEGEDEEENKKKKEAEKNDLTGSAVMNYISKQLKDI
QEFIENTKENIEIRNKNISELINVMINLEKVRVKNDDDVLTLDTVEEMLN
FLKKKKDSDIKKCKKLQEEWKNLAKMAQAVKKDIQNPVKTESDKTKENIK
KFEEITLKEYANSLKKESFFIYKTGVSESFKRIGEVKQKVDEFEVQLNQY
EDFARIGSKKLMEQIRTDVSSVEKLWVRIEISEKTMDEYKKMKWGSINSM
DMEDEIKKLRKALTDLRGIDKRSNAFIGITEELKKWATFLPLLGELKDPS
MNSEDGRHWKKLKDLVKKEFDVSQELMLEIIWDLKLFDYKDGIEEITDQA
KQELKMEKALKKIIDFWRDIEFELVQHKNTDIHTLKMSEENFETLEDHQL
QINNMLLSKYVAYFEKEVEKWKYDLGSVYDVVQLLLEVQKTWSFLENLFE
VKRELPNESAQFVGIDKDMKEIMQKGCDIKNCLKFCTIEGMLKRLENIQA
QLKVCEKALNEFLDSKRRAFPRFYFVSVNDLLDILSNGNSPAKINRHMSK
IFQAIDNLQLKEDSSGGRPTLKMISCVGTEEVDFSSPRLLQGKVESYLKD
VIDTMIGTLKSVANSSFKNFQSMTRKEWLKSDPSQITLLVNNIIWSKAVE
DCFLKLQSGDINAMKLFLDESIKQLTELIGMVQGDLSKPLRQKIMCLITI
DTHSRDVVHRLINEHVRKAEEFQWQSQLKFYWVDNDAKIKIADARFVYNY
EYLGNGPRLVITPLTDRIYVTATQALHLKMGCAPAGPAGTGKTETTKDLA
NALAKACYVFNCSSEMNYESMGNIYKGLASSGCWGCFDEFNRLLPEVLSV
CSVQFKAVTDAIKQNVERFIIEGDEISLDPTCGVFITMNPGYLGRAELPE
GLKALFRPITVVVPDLELICENMLMAEGFIEAKILAKKFVTLYMLCRDLL
SKQLHYDWGLRAIKSVLVVAGGFKRSEPEIAEQALLMRALRDFNIPKIAF
QDLYVFHGLLGDLFPGINIKPKKDLDFEKIITDVCIENKLDPDPEFVLKV
VQLSELLAIRHCVFVMGPPGAGKSTTWKILAKAQDKTNKKTTLIDIDPKV
VSTKDFYGYNLPSKEWKDGLFSKMLRSLAEQPDTNPKWICLDGDLDANWI
ESMNSVMDDNKILTLANNERIPLKPHMRALFEIRDLRFATPATVSRAGIL
YISDEVGYQWRSYVKSWIKQEFSQDQEMSKNLDTLFGKYVPDTLDHIKKH
CRFLVPVSPISQVISICKSLQTLLKGDVKNLEYLFVYALIWAIGGALAEK
DSIDYRKDFSTWWKGAWKTAVKFPSKGTIFDYYVDQSGDSSKFVEWSKRL
ENKEFDPQVETMGNITVNTIETLATTEFIKSYLMVKHPSLLIGNSGCGKT
QLAKGILKEIVQAKPENYAYQLINFNYYTDSTYLQGQIEQTLEKKAGRQY
GPPGKVQLIYFIDDLNMPQLDAYDTQTAIALLRQLADYGHFYDVSKLALK
DIINTQVLAAMNPSAGSFFVNPRYQRHFWTISIPFPDNESLSLIYITFLN
GHLKRFKSTIQEYSNIIVRASLMLHQAVTQNFRKTAINFHYEFNLRHMSN
VFQGLLLSDPNKFTEPDKLIKLWIHECERTYGDRLVSTDNLKTYKENIFD
IVKKSFSKFNFSRYFGNNPENLIYCNFIAGINSDRFYDQMPNNEMEKHIS
EALKEYNDNNAFMGLVLFEDAMKHVCRICRIVLPSSGHALLVGVGGSGKQ
SLSKLASFIMGYTTFSITISATYSMVDLRNDLQQLYFKCGPKEEGILFLF
TEGQITNERFLVYINDLLSSGEIAELYTLDEKEAMINQVRAKVKGEGKPD
TRENCWNWFIDQVKKNLHMAICFSPVGDMRRRARQFPALVNCTVIDWFQP
WPYEALFNVAKSFLEPVDLGDDKVREAVVKFMPFSFTLVNDLGLKLLEQE
RRYAYTTPKSFLELISLFTNMLAQKRESLERNKERYETGLVKLKETAEQV
AIIEVEVKEKQVEAEAKKKEADAFAEVVGREKDKVEKENSKATIEADKCG
LIKQNVEAQKSSTQQDLDAAQPLVEQAKSALNSISKKDFQQAKSFASPPA
GVPEVFAATIYLLAGYFNEAIEIDKNKKPKDVSWKSSLKLMKSPEEFMEK
LLNFKDVVDANQVANVNIVKNQYLNMPSFTPEQMASKSAAAKGICSWVVN
IVKYYDVIQDVEPKRKALKEATEQLEEATVKLNEVEEVVRKLNEELNKLK
AENDKAIAERNAAISEAERCARRLNLAQRLVTALSSENERWGKSIIQLED
QLKLMVGDVLVASSFVSYSGPFNKKFRNIMINQNFMKFMKEHTIPMSPDP
NPIKILTDESTIALWNKQKLPSDSVSIENGTILTNSARYPLMIDPQLQGI
TWIREKEKANNLKILRLGSKNINRDLELSIENGYSAIIENMNERIDAILM
PIIARSFIKRGKNKIIKFAGKDLILHPNFKLFLHTKLSNPHYPPEIQAEA
ALINFTVTEAGLGDQLLSLVVARERPDLAKMKIELITQQNDFKIKLKDLE
DELLYKLANAKGDILDDIELIENLEYSKKLSVEIAEKVAAAKITEAKINE
TSENYRPAASRGALFYFLLSDLSKVHSFYKYSLESFIVVINRAIDAISEN
KIYGKTTMMSPRSLKKRVDELIESLTYTAYQTTRRGLFESHKLIVAAMLC
LRVLLRSEELNSDEVDHLIIGKVDVNPTPMPDALKSFLNDNIWAACKALE
TIHQFQGFCQSLETDVLQWKKWYSEEKAETADLPKAFKELSKFHRLLLLR
ALRPDRLPSALSQFVHDKMGERYIEQPPFNIFETFQETSKTVPIFFVLFP
GVDPTPDVERVAATFDVSANNGRFINISMGQGQEDRAKKALFDCAQKGHW
IMLQNVHLMQSWLYGLNGLEGFLESVFASPKTHPNFRVFISSEPPNVLLP
LMQIIPESILQGSLKIANEAPQYLKANLRRAYNKFDQEFLDKCDKKPTEF
KSCLFALCFFHSLMLGRKKFGTQGWSRVYNFNDGDLTICADVLYNYLSKY
DQVPWDDLRYIFGEIMYGGHITDDWDRRTNRTYLKVLIRSELLQQNFNLA
PQFKSPDPSKFDYEAYRKYIEEKLPIESPQMFGMHPNAEIGYLTQTCDQV
FNTILEVQGGSSGGGASKKDDGVMVTLTDFKTRCPHDFNMLLIEEKVKEK
TPYIVVCLQECERMNGLLKEIKTSLEDLRLGLTGALNMTDAMESLQQSLS
FNKVPDTWEKKAYFSKKPLSSWFADLIERNIQLQEWCKELVTPTSLCISY
LFNPMSYLTAIMQFTARAQGLPLDGITIQTNVTAMKGPEDVVNPAENGAY
IHGLFLEGAAWEIGGQGQDGYLIEQKPKELHPKMPVINAVAVPLDKKKKN
GQYDCPTYVTSARGQTFVFTANLNMESDDSDPNKWILSGTCMLMSDD

3D structure

• PDB: 8bx8 ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.
• Chain: C
• Resolution: 30.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	C	L1657 D1658 F1663 G1687 A1688 G1689 K1690 S1691 T1692 Y1830 R2140 R2143	L1540 D1541 F1546 G1570 A1571 G1572 K1573 S1574 T1575 Y1713 R2023 R2026
BS02	ADP	C	T1983 G2013 C2014 K2016 Q2018 R2213 N2508	T1866 G1896 C1897 K1899 Q1901 R2096 N2391
BS03	ADP	C	G2363 G2365 K2366 Q2367 S2368	G2246 G2248 K2249 Q2250 S2251

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005874 microtubule
• GO:0030286 dynein complex
• GO:0031514 motile cilium

External links

• PDB: RCSB:8bx8, PDBe:8bx8, PDBj:8bx8
• PDBsum: 8bx8
• PubMed: 37051721
• UniProt: I7M6H4