Structure of PDB 8beo Chain C

Receptor sequence

>8beoC (length=592) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHV
EGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCIT
GQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMR
SGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLI
QAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGCIPDDHELM
AGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHI
DIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVAD
CQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTAGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQF
LIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINS
SEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVV
EVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE

3D structure

PDB

8beo Glyoxylate carboligase lacks the canonical active site glutamate of thiamine-dependent enzymes.

Chain

Resolution

1.96 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

4.1.1.47: tartronate-semialdehyde synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	MG	C	D446 N473 Y475	D445 N472 Y474
BS02	FAD	C	R154 P155 G211 G212 G213 N216 T237 L238 M239 V255 G256 Q258 T259 G278 N279 R280 A282 R284 H285 D302 I303 Q307 D321 A322 G416	R153 P154 G210 G211 G212 N215 T236 L237 M238 V254 G255 Q257 T258 G277 N278 R279 A281 R283 H284 D301 I302 Q306 D320 A321 G415
BS03	TDK	C	G394 L395 S396 L421 G445 D446 F447 D448 N473 Y475 L476 G477 L478 I479	G393 L394 S395 L420 G444 D445 F446 D447 N472 Y474 L475 G476 L477 I478
BS04	UQ0	C	E249 Q353 C588	E248 Q352 C587
BS05	MG	C	F451 L452 E454	F450 L451 E453
BS06	ALU	C	H45 F462	H44 F461
BS07	TDK	C	G27 A28 V51 T75 Q115	G26 A27 V50 T74 Q114

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0009028	tartronate-semialdehyde synthase activity
GO:0016829	lyase activity
GO:0030976	thiamine pyrophosphate binding
GO:0042802	identical protein binding
GO:0050660	flavin adenine dinucleotide binding
GO:0071949	FAD binding

	Biological Process
GO:0009097	isoleucine biosynthetic process
GO:0009099	L-valine biosynthetic process
GO:0009436	glyoxylate catabolic process
GO:0019752	carboxylic acid metabolic process
GO:0046296	glycolate catabolic process

	Cellular Component
GO:0005948	acetolactate synthase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8beo, PDBe:8beo, PDBj:8beo
PDBsum	8beo
PubMed
UniProt	P0AEP7\|GCL_ECOLI Glyoxylate carboligase (Gene Name=gcl)

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