Structure of PDB 8bcx Chain C

Receptor sequence

>8bcxC (length=443) Species: 269799 (Geobacter metallireducens GS-15)

KTVQIPDGEVDPAVWGKAYPTEYEMWKKKRGFDADHVTYDKLSEFPYMAL
LFNGWGFGIAYNEPRGHANMVRDQLEIDSARLKSGGVCLTCKTPYAPKLE
KEMGIDYFKTPFKDVLAKIPEKHKTLGVACIDCHDNKDMSLRISRGFTLG
EALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYFPWQGS
KMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHW
KAGAACTDCHMPYTRVGAFKVSDHRVMSPLKNDMKACIQCHTEKPEWLRD
QVIAIQDRTVSLMLRSGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKD
LYEEAFYRCVFIGAENSVGFHNPTEAMRVLGDATAFATKAEALLRQALAK
AGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKDPYGVQVRF

3D structure

• PDB: 8bcx An unusual active site architecture in cytochrome c nitrite reductase NrfA-1 from Geobacter metallireducens.
• Chain: C
• Resolution: 1.941 Å

Enzymatic activity

• Enzyme Commision number: 1.7.2.2: nitrite reductase (cytochrome; ammonia-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	C	H294 A297 C304 C332 C335 H336 F415	H249 A252 C259 C287 C290 H291 F370
BS02	HEC	C	R75 H222 F288 H294 C301 C304 H305 S323 P324 L325 F415 H416	R30 H177 F243 H249 C256 C259 H260 S278 P279 L280 F370 H371
BS03	HEC	C	D80 E108 R110 L120 C133 C136 K137 C221 H222 Y225 H282 E284	D35 E63 R65 L75 C88 C91 K92 C176 H177 Y180 H237 E239
BS04	HEC	C	R75 R110 G111 H112 M115 C136 V173 V217 C218 C221 H222 H305	R30 R65 G66 H67 M70 C91 V128 V172 C173 C176 H177 H260
BS05	HEC	C	Y52 E55 W59 A174 C175 C178 H179 M184 L186 R214 M306 V316 S317 H319	Y19 E22 W26 A129 C130 C133 H134 M139 L141 R169 M261 V271 S272 H274

Gene Ontology

Molecular Function

• GO:0042279 nitrite reductase (cytochrome, ammonia-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:8bcx, PDBe:8bcx, PDBj:8bcx
• PDBsum: 8bcx
• PubMed: 37460131
• UniProt: Q39YY5