Structure of PDB 7ywv Chain C

Receptor sequence

>7ywvC (length=524) Species: 101510 (Rhodococcus jostii RHA1)

RTLPPGVSDERFDAALQRFRDVVGDKWVLSTADELEAFRDPYPVGAAEAN
LPSAVVSPESTEQVQDIVRIANEYGIPLHPVSTGKNNGYGGAAPRLSGSV
IVKTGERMNRILEVNEKYGYALLEPGVTYFDLYEYLQSHDSGLMLDCPEL
GWGSVVGNTLDRGVGYTPYGDHFMWQTGLEVVLPQGEVMRTGMGALPGSD
AWQLFPYGFGPFPDGMFTQSNLGIVTKMGIALMQRPPASQSFLITFDKEE
DLEQIVDIMLPLRINMAPLQNVPVLRNIFMDAAAVSKRTEWFDGDGPMPA
EAIERMKKDLDLGFWNFYGTLYGPPPLIEMYYGMIKEAFGKIPGARFFTH
EERDDRGGHVLQDRHKINNGIPSLDELQLLDWVPNGGHIGFVPVSAPDGR
EAMKQFEMVRNRANEYNKDYMASFIIGLREMYHVCLFIYDTADPEAREEI
LQMTKVLVREAAEAGYGEYRTHNALMDDVMATFNWGDGALLKFHEKIKDA
LDPNGIIAPGKSGIWPQRFRGQNL

3D structure

• PDB: 7ywv Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound.
• Chain: C
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.38: vanillyl-alcohol oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	C	P82 V83 S84 T85 G86 K87 N88 N89 P150 E151 L152 G155 S156 G159 N160 L162 G165 V166 Y168 I226 V227 H390 R472	P80 V81 S82 T83 G84 K85 N86 N87 P148 E149 L150 G153 S154 G157 N158 L160 G163 V164 Y166 I224 V225 H388 R470
BS02	EOL	C	Y91 E151 Y168 V436 Y471	Y89 E149 Y166 V434 Y469

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004458 D-lactate dehydrogenase (cytochrome) activity
• GO:0008720 D-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0018465 vanillyl-alcohol oxidase activity
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:1903457 lactate catabolic process

External links

• PDB: RCSB:7ywv, PDBe:7ywv, PDBj:7ywv
• PDBsum: 7ywv
• PubMed: 36418310
• UniProt: Q0SBK1